2pox

Dark state structure of the reversibly switchable fluorescent protein Dronpa

spinqualitylabelsall models 2pox, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2pox is a 4 chain structure with sequence from Echinophyllia sp. sc22. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: GYC Related: 2iov, 2ie2 Gene: Dronpa (Echinophyllia sp. SC22) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Dronpa is a novel GFP-like fluorescent protein with exceptional light-controlled switching properties. It may be reversibly switched between a fluorescent on-state and a nonfluorescent off-state by irradiation with light. To elucidate the molecular basis of the switching mechanism, we generated reversibly switchable Dronpa protein crystals. Using these crystals we determined the elusive dark-state structure of Dronpa at 1.95-A resolution. We found that the photoswitching results in a cis-trans isomerization of the chromophore accompanied by complex structural rearrangements of four nearby amino acid residues. Because of this cascade of intramolecular events, the chromophore is exposed to distinct electrostatic surface potentials, which are likely to influence the protonation equilibria at the chromophore. We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore. Structural basis for reversible photoswitching in Dronpa.,Andresen M, Stiel AC, Trowitzsch S, Weber G, Eggeling C, Wahl MC, Hell SW, Jakobs S Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):13005-9. Epub 2007 Jul 23. PMID:17646653 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Dronpa Green Fluorescent Protein

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