2shk: Difference between revisions
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|PDB= 2shk |SIZE=350|CAPTION= <scene name='initialview01'>2shk</scene>, resolution 2.6Å | |PDB= 2shk |SIZE=350|CAPTION= <scene name='initialview01'>2shk</scene>, resolution 2.6Å | ||
|SITE= <scene name='pdbsite=PIP:A+Walker+Type-A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>PIP</scene>, <scene name='pdbsite=POP:A+Walker+Type-A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>POP</scene>, <scene name='pdbsite=SAS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SAS</scene> and <scene name='pdbsite=SBS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SBS</scene> | |SITE= <scene name='pdbsite=PIP:A+Walker+Type-A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>PIP</scene>, <scene name='pdbsite=POP:A+Walker+Type-A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>POP</scene>, <scene name='pdbsite=SAS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SAS</scene> and <scene name='pdbsite=SBS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SBS</scene> | ||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ADP:ADENOSINE-5 | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71] | |ACTIVITY= [http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71] | ||
|GENE= AROL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi]) | |GENE= AROL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi]) | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:50:31 2008'' | ||
Revision as of 13:50, 23 March 2008
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| 2shk, resolution 2.6Å | |||||||||||||
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| Sites: | PIP, POP, SAS and SBS | ||||||||||||
| Ligands: | MG and ADP | ||||||||||||
| Gene: | AROL (Erwinia chrysanthemi) | ||||||||||||
| Activity: | Shikimate kinase, with EC number 2.7.1.71 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI COMPLEXED WITH ADP
Overview
Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia coli has been crystallized by the vapour-diffusion method using sodium chloride as a precipitant. Mass spectrometry was used to confirm the purity of the shikimate kinase and dynamic light scattering was used to assess conditions for the monodispersity of the enzyme. The crystals are tetragonal, space group P4(1)2(1)2 or enantiomorph with cell dimensions a = b = 108.5 and c = 92.8 A (at 100 K). Native crystals diffract to better than 2.6 A on a synchrotron X-ray source. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 3.6 A(3) Da(-1).
About this Structure
2SHK is a Single protein structure of sequence from Erwinia chrysanthemi. Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:15299895
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