1ef4: Difference between revisions
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|PDB= 1ef4 |SIZE=350|CAPTION= <scene name='initialview01'>1ef4</scene> | |PDB= 1ef4 |SIZE=350|CAPTION= <scene name='initialview01'>1ef4</scene> | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK04016 PRK04016]</span> | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ef4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ef4 OCA], [http://www.ebi.ac.uk/pdbsum/1ef4 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1ef4 RCSB]</span> | |||
}} | }} | ||
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[[Category: Mcintosh, L P.]] | [[Category: Mcintosh, L P.]] | ||
[[Category: NESG, Northeast Structural Genomics Consortium.]] | [[Category: NESG, Northeast Structural Genomics Consortium.]] | ||
[[Category: nesg]] | [[Category: nesg]] | ||
[[Category: northeast structural genomics consortium]] | [[Category: northeast structural genomics consortium]] | ||
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[[Category: zinc binding]] | [[Category: zinc binding]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 05:51:35 2008'' | ||
Revision as of 03:51, 26 March 2008
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| 1ef4 | |||||||||||||
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| Ligands: | ZN | ||||||||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||||||||
| Domains: | PRK04016 | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
SOLUTION STRUCTURE OF THE ESSENTIAL RNA POLYMERASE SUBUNIT RPB10 FROM METHANOBACTERIUM THERMOAUTOTROPHICUM
Overview
The RNA polymerase subunit RPB10 displays a high level of conservation across archaea and eukarya and is required for cell viability in yeast. Structure determination of this RNA polymerase subunit from Methanobacterium thermoautotrophicum reveals a topology, which we term a zinc-bundle, consisting of three alpha-helices stabilized by a zinc ion. The metal ion is bound within an atypical CX(2)CX(n)CC sequence motif and serves to bridge an N-terminal loop with helix 3. This represents an example of two adjacent zinc-binding Cys residues within an alpha-helix conformation. Conserved surface features of RPB10 include discrete regions of neutral, acidic, and basic residues, the latter being located around the zinc-binding site. One or more of these regions may contribute to the role of this subunit as a scaffold protein within the polymerase holoenzyme.
About this Structure
1EF4 is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA.
Reference
Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum., Mackereth CD, Arrowsmith CH, Edwards AM, McIntosh LP, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6316-21. PMID:10841539
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Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- DNA-directed RNA polymerase
- Methanothermobacter thermautotrophicus
- Single protein
- Arrowsmith, C H.
- Edwards, A M.
- Mackereth, C D.
- Mcintosh, L P.
- NESG, Northeast Structural Genomics Consortium.
- Nesg
- Northeast structural genomics consortium
- Protein structure initiative
- Psi
- Structural genomic
- Three helix bundle
- Zinc binding
