1w2w: Difference between revisions

Ypr118w is a non-essential, low copy number gene product from, Saccharomyces cerevisiae. It belongs to the PFAM family PF01008, which, contains the alpha-, beta-, and delta-subunits of eukaryotic translation, initiation factor eIF2B, as well as proteins of unknown function from all, three kingdoms. Recently, one of those latter proteins from Bacillus, subtilis has been characterized as a 5-methylthioribose-1-phosphate, isomerase, an enzyme of the methionine salvage pathway. We report here the, crystal structure of Ypr118w, which reveals a dimeric protein with two, domains and a putative active site cleft. The C-terminal domain resembles, ribose-5-phosphate isomerase from Escherichia coli with a similar location, of the active site. In vivo, Ypr118w protein is required for yeast cells, to grow on methylthioadenosine in the absence of methionine, showing that, Ypr118w is involved in the methionine salvage pathway. The crystal, structure of Ypr118w reveals for the first time the fold of a PF01008, member and allows a deeper discussion of an enzyme of the methionine, salvage pathway, which has in the past attracted interest due to tumor, suppression and as a target of aniprotozoal drugs.

1W2W is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/S-methyl-5-thioribose-1-phosphate_isomerase S-methyl-5-thioribose-1-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.23 5.3.1.23] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W2W OCA].  

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