4p0z

Structure of the double stranded DNA binding type IV secretion protein TraN from Enterococcus

spinqualitylabelsall models 4p0z, resolution 1.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4p0z is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: MSE Related: 4edd Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Publication Abstract from PubMed Conjugative transfer through type IV secretion multiprotein complexes is the most important means of spreading antimicrobial resistance. Plasmid pIP501, frequently found in clinical Enterococcus faecalis and Enterococcus faecium isolates, is the first Gram-positive (G+) conjugative plasmid for which self-transfer to Gram-negative (G-) bacteria has been demonstrated. The pIP501-encoded type IV secretion system (T4SS) protein TraN localizes to the cytoplasm and shows specific DNA binding. The specific DNA-binding site upstream of the pIP501 origin of transfer (oriT) was identified by a novel footprinting technique based on exonuclease digestion and sequencing, suggesting TraN to be an accessory protein of the pIP501 relaxase TraA. The structure of TraN was determined to 1.35 A resolution. It revealed an internal dimer fold with antiparallel beta-sheets in the centre and a helix-turn-helix (HTH) motif at both ends. Surprisingly, structurally related proteins (excisionases from T4SSs of G+ conjugative transposons and transcriptional regulators of the MerR family) resembling only one half of TraN were found. Thus, TraN may be involved in the early steps of pIP501 transfer, possibly triggering pIP501 TraA relaxase activity by recruiting the relaxosome to the assembled mating pore. Structure of the double-stranded DNA-binding type IV secretion protein TraN from Enterococcus.,Goessweiner-Mohr N, Eder M, Hofer G, Fercher C, Arends K, Birner-Gruenberger R, Grohmann E, Keller W Acta Crystallogr D Biol Crystallogr. 2014 Sep 1;70(Pt 9):2376-89. doi:, 10.1107/S1399004714014187. Epub 2014 Aug 29. PMID:25195751 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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