197d: Difference between revisions
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|PDB= 197d |SIZE=350|CAPTION= <scene name='initialview01'>197d</scene>, resolution 2.190Å | |PDB= 197d |SIZE=350|CAPTION= <scene name='initialview01'>197d</scene>, resolution 2.190Å | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=197d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=197d OCA], [http://www.ebi.ac.uk/pdbsum/197d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=197d RCSB]</span> | |||
}} | }} | ||
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[[Category: double helix]] | [[Category: double helix]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:30:09 2008'' | ||
Revision as of 15:30, 30 March 2008
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| 197d, resolution 2.190Å | |||||||||||||
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| Ligands: | DA, DC, DG, DT | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
ORTHORHOMBIC CRYSTAL STRUCTURE OF THE A-DNA OCTAMER D(GTACGTAC). COMPARISON WITH THE TETRAGONAL STRUCTURE
Overview
The X-ray crystal structure of the double-helical A-DNA octanucleotide d(GTACGTAC) has been solved by molecular replacement and refined to a resolution of 0.219 nm. The final R-factor is equal to 16.1% for 1516 observed reflections with F > 4 sigma(F). The sequence crystallizes as an A-DNA-type double helix in the orthorhombic space group P2(1)2(1)2, with one duplex molecule solvated by 66 water molecules in the asymmetric unit. Cell parameters are a = 3.860 nm, b = 5.082 nm, c = 2.174 nm. It is the first time that such a crystal form has been observed. This orthorhombic structure has been compared with the tetragonal structure of the same oligonucleotide. It adopts a bent structure with an unusual packing between symmetry-related molecules.
About this Structure
197D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Orthorhombic crystal structure of the A-DNA octamer d(GTACGTAC). Comparison with the tetragonal structure., Langlois d'Estaintot B, Dautant A, Courseille C, Precigoux G, Eur J Biochem. 1993 Apr 15;213(2):673-82. PMID:8477741
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