1ogv: Difference between revisions
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==Overview== | ==Overview== | ||
Well-ordered crystals of the bacterial photosynthetic reaction centre from, Rhodobacter sphaeroides were grown from a lipidic cubic phase. Here, we, report the type I crystal packing that results from this crystallisation, medium, for which 3D crystals grow as stacked 2D crystals, and the, reaction centre X-ray structure is refined to 2.35A resolution. In this, crystal form, the location of the membrane bilayer could be assigned with, confidence. A cardiolipin-binding site is found at the protein-protein, interface within the membrane-spanning region, shedding light on the, formation of crystal contacts within the membrane. A chloride-binding site, was identified in the membrane-spanning region, which suggests a putative, site for interaction with the light-harvesting complex I, the .. | Well-ordered crystals of the bacterial photosynthetic reaction centre from, Rhodobacter sphaeroides were grown from a lipidic cubic phase. Here, we, report the type I crystal packing that results from this crystallisation, medium, for which 3D crystals grow as stacked 2D crystals, and the, reaction centre X-ray structure is refined to 2.35A resolution. In this, crystal form, the location of the membrane bilayer could be assigned with, confidence. A cardiolipin-binding site is found at the protein-protein, interface within the membrane-spanning region, shedding light on the, formation of crystal contacts within the membrane. A chloride-binding site, was identified in the membrane-spanning region, which suggests a putative, site for interaction with the light-harvesting complex I, the cytochrome, bc(1) complex or PufX. Comparisons with the X-ray structures of this, reaction centre deriving from detergent-based crystals are drawn, indicating that a slight compression occurs in this lipid-rich, environment. | ||
==About this Structure== | ==About this Structure== | ||
1OGV is a | 1OGV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with FE2, CL, BCL, BPH, U10 and CDL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OGV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:03:43 2007'' | ||
