4uei: Difference between revisions
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''' | ==Solution structure of the sterol carrier protein domain 2 of Helicoverpa armigera== | ||
<StructureSection load='4uei' size='340' side='right' caption='[[4uei]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4uei]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UEI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UEI FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uei OCA], [http://pdbe.org/4uei PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uei RCSB], [http://www.ebi.ac.uk/pdbsum/4uei PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The cotton bollworm, Helicoverpa armigera, has developed strong resistance to many insecticides. Sterol Carrier Protein-2 (SCP-2) is an important non-specific lipid transfer protein in insects and appears to be a potential new target. In order to elucidate the structure and function of Helicoverpa armigera SCP-2 (HaSCP-2), NMR spectroscopy, docking simulations, mutagenesis and bioassays were performed. HaSCP-2 composed of five alpha-helices and four stranded beta-sheets. The folds of alpha-helices and beta-sheets interacted together to form a hydrophobic cavity with putative entrance and exit openings, which served as a tunnel for accommodating and transporting of lipids. Several sterols and fatty acids could interact with HaSCP-2 via important hydrophobic sites, which could be potential targets for insecticides. Mutagenesis experiments indicated Y51, F53, F89, F110, I117 and Q131 may be the key functional sites. HaSCP-2 showed high cholesterol binding activity and SCP-2 inhibitors (SCPIs) could inhibit the biological activity of HaSCP-2. SCPI-treated larvae at young stage showed a significant decrease of cholesterol uptake in vivo. Our study describes for the first time a NMR structure of SCP-2 in lepidopteran H. armigera and reveals its important function in cholesterol uptake, which facilitates the screening of effective insecticides targeting the insect cholesterol metabolism. | |||
NMR structure and function of Helicoverpa armigera sterol carrier protein-2, an important insecticidal target from the cotton bollworm.,Ma H, Ma Y, Liu X, Dyer DH, Xu P, Liu K, Lan Q, Hong H, Peng J, Peng R Sci Rep. 2015 Dec 10;5:18186. doi: 10.1038/srep18186. PMID:26655641<ref>PMID:26655641</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4uei" style="background-color:#fffaf0;"></div> | |||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Hong, H]] | |||
[[Category: Liu, X]] | |||
[[Category: Ma, H]] | |||
[[Category: Peng, J]] | |||
[[Category: Peng, R]] | [[Category: Peng, R]] | ||
[[Category: Yan, X]] | [[Category: Yan, X]] | ||
[[Category: | [[Category: Insecticidal target]] | ||
[[Category: | [[Category: Scp-2]] | ||
[[Category: | [[Category: Transferase]] | ||
