Calmodulin

spinqualitylabelsall models Human calmodulin complex with ethanol and Ca+2 ions (green) (PDB code 1cll) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Calmodulin (CaM) – calcium modulated protein – regulates various protein targets. It is used by various proteins as calcium sensor and signal transducer by binding to their calcium binding domain (CBD). It undergoes conformational change upon binding Ca++ via its 4 EF hand motives and can undergo post-translational modification. Click here to see EF hand of Human calmodulin (PDB code 1cll). More details on apo-CaM in Calcium-free Calmodulin and Calmodulin JMU. Maximum Occurrence of Calmodulin Conformations Maximum Occurrence, a method for making rigorous numerical assessments about the maximum percent of time that a conformer of a flexible macromolecule can exist and still be compatible with the experimental data, was used to probe the conformational disorder of Calmodulin. Figure 3: Orientation tensor representation for 400 conformational states of Calmodulin, color coded according to their MO values (from less than 5% in blue to more than 30% in red).To better explain their meaning, 10 randomly chosen models are shown as cartoons and then replaced by the three axes of their color-coded orientation tensors. It was shown that the open (1cll) and closed (1prw) conformers can have MO of only 15% and 5% respectively. Calmodulin in Motion The clip represents Calmodulin in motion. At the beginning it is shown moving in the unbound form (ApoCaM), and it changes its conformation when Calcium ions are present in the medium (CaCaM). <swf width="720" height="576">/flash/Apo_CaM_CaCaM.swf</swf> Motion of ApoCaM is elaborated on the basis of 23 conformations derived from NMR file 1cfc, using the 3D animation program Blender, and according to a system to be published soon (Zini et al., manuscript in preparation). The transition from ApoCaM to CaCaM is elaborated with Blender starting with conformation 21 of 1cfc to arrive in conformation 11 of pdb file 1x02. Surface rendering is also elaborated using Blender, and shows the lipophilic potential as a scale of white-black and smooth-rough, form the most lipophilic to the hydrophilic. Electrostatic potential is represented as a series of lines moving in the direction Positive to Negative, elaborated according to a scheme to be published soon (Andrei et al., in preparation). As most lines are moving towards Calmodulin, one can learn that the protein is slightly acidic (negative partial charges on its surface). This movie was created by Andrei, Zini et al., of the Scientific Visualization Unit, Institute of Clinical Physiology - CNR of Itlay. Conformational change of Calmodulin Unbound, Bound

3D Structures of Calmodulin

Updated on 05-January-2016

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• Native CaM
  • EF hand, 1cll – bCaM - bovine
    
  • Calcium-free Calmodulin – cCaM – chicken
    
  • Calmodulin JMU, Maximum Occurrence, Maximum Occurrence – PtCaM - Paramecium tetraurelia
    
  • 1cll – rCaM - rat
    
  • 1prw, 1cfc – XlCaM – NMR - Xenopus laevis
    
  • 2k61, 2k0e – hCaM – NMR - human
    
  • 1y6w, 1cll, 4bw8 - hCaM
    
  • 4cln – DmCaM - Drosophila melanogaster
    
  • 1rfj – CaM – potato
    
  • 1ooj – CaM – Caenorhabditis elegans
    
  • 4hex – mCaM – mouse
    
• Mutant CaM
  • 1ahr, 2m3s – cCaM (mutant)
    
  • 2k0j, 1sw8 – hCaM (mutant) – NMR
    
• apo CaM
  • 1lkj – yapoCaM – NMR -yeast
    
  • 1cfc, 1cfd - XlapoCaM – NMR
    
  • 1qx5 – rapoCaM – rat
    
• CaM N-terminal
  • 2i08 - hCaM N-terminal (mutant)
    
  • 1j7o, 1j7p - hCaM N-terminal – NMR
    
  • 1f70 - XlCaM N-terminal – NMR
    
  • 2ro8, 2roa - sCaM N-terminal+Ca – NMR - soybean
    
  • 2ro9, 2rob - sCaM C-terminal+Ca – NMR
    
  • 3ifk, 3b32 – rCaM N-terminal
    
  • 1f54, 1f55 – yapoCaM N-terminal – NMR
    
• CaM C-terminal
  • 1f71 - XlCaM C-terminal – NMR
    
  • 1cmg – bCaM C-terminal – NMR
    
  • 1fw4 - bCaM C-terminal
    
  • 1cmf – bapoCaM C-terminal – NMR
    
  • 2hf5 - hCaM EF2 EF3 – NMR
    
  • 2lqp - hCaM EF3 EF4 – NMR
    
  • 2kxw – PtapoCaM + IQ motif of NAV1.2 – NMR
    
  • 2m5e – PtCaM + Na channel protein
    
  • 2kz2, 4bya – CaM (mutant) – chicken - NMR
    
  • 2rrt – XlCaM (mutant)
• CaM+ cations (non calcium)
  • 2ksz – sCaM N-terminal+Mg – NMR
    
  • 1ak8 - bCaM N-terminal+Ce – NMR
    
  • 2eqc – XlCaM C-terminal+Mg– NMR
    
  • 2pq3 – rCaM+Zn
    
  • 2v01 – hCaM+Pb
    
  • 2v02 – hCaM+Ba
    
  • 4bw7 – hCaM + Sr
    
  • 3uct – hCaM + Mn + Zn
    
  • 3ucw - hCaM N-terminal + Mg
    
  • 3ucy - hCaM N-terminal + Mg + Zn
    
  • 1n0y – PtCaM+Pb
    
  • 2lhh – yCaM + Ca - NMR
• CaM small molecule complexes
  • 3if7 – bCaM+sphingosylphosphorylcholine
    
  • 1qiv, 1qiw – bCaM+DPDv
    
  • 1a29, 1lin – bCaM+trifluoperazine
    
  • 1ctr - hCaM+trifluoperazine
    
  • 2kug, 2kuh - hCaM N-terminal EF1 EF2+halothane – NMR
    
  • 2kdu – XlCaM+MUNC13-1 – NMR
    
  • 1mux – XlCaM+W-7 – NMR
    
  • 1xa5 – bCaM+KAR-2
    
• CaM complexed with protein CBD domains
  • 3gp2 – cCaM+CaM kinase II δ chain
    
  • 2dfs – cCaM+myosin 5A
    
  • 2o5g – cCaM+ myosin light chain kinase peptide
    
  • 2bcx – cCaM+ryanodine receptor 1 peptide
    
  • 3gof, 2o60 – cCaM+nitric oxide synthase CBD
    
  • 1niw - rCaM+nitric oxide synthase CBD
    
  • 2hqw - rCaM+glutamate receptor peptide
    
  • 3bxk, 3bxl, 4ehq, 4j9y, 4j9z – rCaM+ calcium channel peptide
    
  • 1g4y, 3sjq, 4g27, 4g28, 4qnh - rCaM+ potassium channel CBD
    
  • 2ygg – rCaM + Na/H exchanger CBD
    
  • 2mgu – rCaM + HIV-1 matrix protein CBD – NMR
    
  • 1qx7 – rapoCaM+potassium channel peptide
    
  • 4gow, 4umo, 4v0c – hCaM + potassium channel peptide
    
  • 3hr4, 2ll6, 2ll7 - hCaM+nitric oxide synthase CBD
    
  • 1yr5, 1wrz, 2y4v – hCaM+death-associated protein kinase 1 (DAP)
    
  • 2l7l - hCaM+ CaM-dependent protein kinase 1 CBD
    
  • 2m0j, 2m0k - hCaM+olfactory channel peptide
    
  • 2kne – hCaM+PMCA C-terminal CBD
    
  • 4upu - hCaM+ IP3 3-K CBD
    
  • 3ewt, 3ewv – hCaM+TNFR fragment
    
  • 2w73, 2jzi, 2r28 – hCaM+Ser/Thr phosphatase CBD
    
  • 3bya – hCaM+glutamate receptor peptide
    
  • 3g43, 2vay, 2f3y, 2f3z, 2be6, 2lqc, 3oxq – hCaM+calcium channel CAV1.2
    
  • 3dve, 3dvj, 3dvk, 3dvm - hCaM+calcium channel CAV2.2
    
  • 2k0f, 2lv6 – hCaM+myosin light chain kinase peptide – NMR
    
  • 1zuz – hCaM+DRP kinase peptide
    
  • 1l7z – hCaM+CAP-23/NAP-22 CBD
    
  • 1cdl - hCaM+CaM dependent kinase CBD
    
  • 1iwq - hCaM+MARCKS CBD
    
  • 3sui – hCaM + capsaicin receptor peptide
    
  • 2m55 – hCaM + α-synuclein peptide
    
  • 2kn2 – sCaM C-terminal+NtMKP1 CBD – NMR
    
  • 1cff - XlCaM+ calcium channel CBD - NMR
    
  • 1sy9 – XlCaM+olfactory channel peptide
    
  • 1nwd – XlCaM+glutamate decarboxylase CBD
    
  • 1iq5 – XlCaM+CaM dependent kinase CBD
    
  • 1ckk - XlCaM+CaM dependent kinase CBD - NMR
    
  • 2llo, 2llq – XlCaM EF-hand domain + estrogen receptor CBD – NMR
    
  • 2mes – XlCaM +PSD95 peptide - NMR
    
  • 2mg5 - XlCaM+nitric oxide synthase peptide - NMR
    
  • 2bbm, 2bbn - DmCaM+myosin light chain kinase peptide
    
  • 1mxe – DmCaM+rCaMKI CBD
    
  • 2fot - bCaM+α-II spectrin CBD
    
  • 2f2o, 2f2p – bCaM+calcium
    
  • 1xa5 – bCaM+KAR-2
    
  • 1cm1, 1cm4, 1cdm - bCaM+CaM dependent kinase CBD
    
  • 4e50 – mCam/linker/neurogranin IQ motif
    
  • 4e53 – mCam/linker/neuromoduin IQ motif
    
  • 4aqr – CaM-7 + calcium-transporting ATPase peptide – Arabidopsis thaliana
    
• Calmodulin complex with protein
  • 2col, 1yrt, 1yru – BpCaM+adenyl cyclase – Bordetella pertussis
    
  • 1zot – BpCaM C-terminal+adenyl cyclase
    
  • 1xfu, 1xfv, 1xfw, 1xfx, 1xfy, 1xfz, 1y0v, 1sk6, 1pk0, 1lvc, 1k90, 1k93 - CaM+adenyl cyclase – Bacillus anthracis
    
  • 2l1w – CaM+vacuolar calcium ATPase peptide – soybean – NMR
    
  • 2lgf – hCaM + selectin peptide
    
  • 3j41 – hCaM + lens fiber major intrinsic protein – Cryo EM
    
  • 2vas, 2vb6, 3gn4, 3l9i, 4dbp, 4dbq, 2x51, 4anj – DmCaM+pMyosin IV
    
  • 1qs7, 1qtx – CaM+RS20 – Escherichia coli
    
  • 1vrk – CaM (mutant)+RS20
    
  • 3ek4, 3ek7, 3ek8, 3ekh, 3ekj, 3evr, 3evu, 3evv, 3o77, 3o78 – CaM/DFP/myosin light chain kinase
    
  • 4ds7 – CaM + spindle pole body component 10 – Kluyveromyces lactis
    
  • 2dfs – cCaM+myosin 5A
    
  • 4l79, 4byf – hCaM + myosin 1B
    
  • 4r8g – rCaM + myosin 1B
    
  • 2x0g – hCaM+death-associated protein kinase 1 (DAP)
    
  • 4djc, 2l53, 4ovn - hCaM+ sodium channel protein type V α subunit
    
  • 4dck, 4jpz - hCaM+ sodium channel protein type V α subunit + fibroblast growth factor 13
    
  • 4jq0 - hCaM+ sodium channel protein type V α subunit + fibroblast growth factor 12
    
  • 2x51, 4anj – DmCaM+pMyosin IV
    
  • 2k3s – CaM+smoothelin-like protein 1 – NMR
    
  • 2ix7 – apoCaM+myosin-5A
    
  • 2bki, 2bkh – CaM+myosin VI – pig
    
  • 4lzx, 4m1l - hCaM+ IQ domain-containing protein G
    
  • 4q57 – hCaM N-terminal + plectin
    
  • 4q5u – hCaM + calcineurin
    

See Also

• Calmodulin at Wikipedia
• Molecule of the Month (08/2003) at RCSB PDB

Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Karsten Theis, Enrico Ravera, Daniel Moyano-Marino, Jaime Prilusky, Michal Harel