1o76: Difference between revisions
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==Overview== | ==Overview== | ||
The crystal structure of the ternary cyanide complex of P450cam and, camphor was determined to 1.8A resolution and found to be identical with, the structure of the active oxygen complex [I. Schlichting et al., 2000, Science 287, 1615]. Notably, cyanide binds in a bent mode and induces the, active conformation that is characterized by the presence of two water, molecules and a flip of the carbonyl of the conserved Asp251. The, structure of the ternary complex of cyanide, L-arginine, and the oxygenase, domain of inducible nitric oxide synthase was determined to 2.4A, resolution. Cyanide binds essentially linearly, interacts with L-Arg, and, induces the binding of a water molecule at the active site. This water is, positioned by backbone interactions, located 2.8A from the nitrogen atom, of . | The crystal structure of the ternary cyanide complex of P450cam and, camphor was determined to 1.8A resolution and found to be identical with, the structure of the active oxygen complex [I. Schlichting et al., 2000, Science 287, 1615]. Notably, cyanide binds in a bent mode and induces the, active conformation that is characterized by the presence of two water, molecules and a flip of the carbonyl of the conserved Asp251. The, structure of the ternary complex of cyanide, L-arginine, and the oxygenase, domain of inducible nitric oxide synthase was determined to 2.4A, resolution. Cyanide binds essentially linearly, interacts with L-Arg, and, induces the binding of a water molecule at the active site. This water is, positioned by backbone interactions, located 2.8A from the nitrogen atom, of cyanide, and could provide a proton required for O-O bond scission in, the hydroxylation reaction of nitric oxide synthase. | ||
==About this Structure== | ==About this Structure== | ||
1O76 is a | 1O76 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with CYN, K, HEM, CAM and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O76 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:12:24 2007'' | ||
