2c19: Difference between revisions
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==Overview== | ==Overview== | ||
Porphobilinogen synthase catalyzes the first committed step of the, tetrapyrrole biosynthesis pathway. In an aldol-like condensation, two, molecules of 5-aminolevulinic acid (ALA) form the first pyrrole, porphobilinogen. Newly synthesized analogues of a reaction intermediate of, porphobilinogen synthase have been employed in studying the active site, and the catalytic mechanism of this early enzyme of tetrapyrrole, biosynthesis. This study combines structural and kinetic evaluation of the, inhibition potency of these inhibitors. In addition, one of the determined, protein structures provides for the first time structural evidence of a, magnesium ion in the active site. From these results, we can corroborate, an earlier postulated enzymatic mechanism that starts with formation of a, C-C .. | Porphobilinogen synthase catalyzes the first committed step of the, tetrapyrrole biosynthesis pathway. In an aldol-like condensation, two, molecules of 5-aminolevulinic acid (ALA) form the first pyrrole, porphobilinogen. Newly synthesized analogues of a reaction intermediate of, porphobilinogen synthase have been employed in studying the active site, and the catalytic mechanism of this early enzyme of tetrapyrrole, biosynthesis. This study combines structural and kinetic evaluation of the, inhibition potency of these inhibitors. In addition, one of the determined, protein structures provides for the first time structural evidence of a, magnesium ion in the active site. From these results, we can corroborate, an earlier postulated enzymatic mechanism that starts with formation of a, C-C bond, linking C3 of the A-side ALA to C4 of the P-side ALA through an, aldole addition. The obtained data are discussed with respect to the, current literature. | ||
==About this Structure== | ==About this Structure== | ||
2C19 is a | 2C19 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with MG and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C19 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: porphyrin biosynthesis]] | [[Category: porphyrin biosynthesis]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:18:10 2007'' | ||
