1vzw: Difference between revisions
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==Overview== | ==Overview== | ||
Some bacterial genomes contain an incomplete set of genes encoding, phosphoribosyl isomerases, raising the question of whether there exists, broadened substrate specificity for the missing gene products. To, investigate the underlying molecular principles of this hypothesis, we, have determined the crystal structure of the bifunctional enzyme PriA from, Streptomyces coelicolor at 1.8 A resolution. It consists of a, (betaalpha)(8)-barrel fold that is assembled by two symmetric, (betaalpha)(4) half-barrels. The structure shows how its active site may, catalyse the isomerization reactions of two different substrates, and we, provide a plausible model of how the smaller of the two substrates could, be bound in two different orientations. Our findings expand the, half-barrel ancestor concept .. | Some bacterial genomes contain an incomplete set of genes encoding, phosphoribosyl isomerases, raising the question of whether there exists, broadened substrate specificity for the missing gene products. To, investigate the underlying molecular principles of this hypothesis, we, have determined the crystal structure of the bifunctional enzyme PriA from, Streptomyces coelicolor at 1.8 A resolution. It consists of a, (betaalpha)(8)-barrel fold that is assembled by two symmetric, (betaalpha)(4) half-barrels. The structure shows how its active site may, catalyse the isomerization reactions of two different substrates, and we, provide a plausible model of how the smaller of the two substrates could, be bound in two different orientations. Our findings expand the, half-barrel ancestor concept by demonstrating that symmetry-related, half-barrels could provide a smart solution to cope with dual substrate, specificity. The data may help to unravel molecular rationales regarding, how organisms with miniature genomes can keep central biological pathways, functional. | ||
==About this Structure== | ==About this Structure== | ||
1VZW is a | 1VZW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor] with SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: CAT. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VZW OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: tryptophan biosynthesis]] | [[Category: tryptophan biosynthesis]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:19:49 2007'' | ||
