1c89: Difference between revisions

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==Overview==

The structure of a new antifreeze protein (AFP) variant, RD3, from, antarctic eel pout (Rhigophila dearborni) with enhanced activity has been, determined for the first time by nuclear magnetic resonance spectroscopy., RD3 comprises a unique translational topology of two homologous type III, AFP globular domains, each containing one flat, ice binding plane. The ice, binding plane of the N domain is located approximately 3.5 A "behind" that, of the C domain. The two ice binding planes are located laterally with an, angle of 32 +/- 12 degrees between the planes. These results suggest that, the C domain plane of RD3 binds first to the ice [1010] prism plane in the, <0001> direction, which induces successive ice binding of the N domain in, the <0101> direction. This manner of ice .~~.. [[http://ispc.weizmann.ac.il/pmbin/getpm?11010977 (full description)]]~~

The structure of a new antifreeze protein (AFP) variant, RD3, from, antarctic eel pout (Rhigophila dearborni) with enhanced activity has been, determined for the first time by nuclear magnetic resonance spectroscopy., RD3 comprises a unique translational topology of two homologous type III, AFP globular domains, each containing one flat, ice binding plane. The ice, binding plane of the N domain is located approximately 3.5 A "behind" that, of the C domain. The two ice binding planes are located laterally with an, angle of 32 +/- 12 degrees between the planes. These results suggest that, the C domain plane of RD3 binds first to the ice [1010] prism plane in the, <0001> direction, which induces successive ice binding of the N domain in, the <0101> direction. This manner of ice binding caused by the unique, structural topology of RD3 is thought to be crucial for the significant, enhancement of antifreeze activity, especially at low AFP concentrations.

==About this Structure==

1C89 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pachycara_brachycephalum Pachycara brachycephalum]]. Structure known Active Sites: IC1 and IC2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C89 OCA]].

1C89 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pachycara_brachycephalum Pachycara brachycephalum]. Structure known Active Sites: IC1 and IC2. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C89 OCA].

==Reference==

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[[Category: thermal hysteresis protein]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on ~~Tue Oct 30 14~~:58:31 2007''

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:25:41 2007''

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@@ Line 5: / Line 5: @@
 ==Overview==
-The structure of a new antifreeze protein (AFP) variant, RD3, from, antarctic eel pout (Rhigophila dearborni) with enhanced activity has been, determined for the first time by nuclear magnetic resonance spectroscopy., RD3 comprises a unique translational topology of two homologous type III, AFP globular domains, each containing one flat, ice binding plane. The ice, binding plane of the N domain is located approximately 3.5 A "behind" that, of the C domain. The two ice binding planes are located laterally with an, angle of 32 +/- 12 degrees between the planes. These results suggest that, the C domain plane of RD3 binds first to the ice [1010] prism plane in the, &lt;0001&gt; direction, which induces successive ice binding of the N domain in, the &lt;0101&gt; direction. This manner of ice ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11010977 (full description)]]
+The structure of a new antifreeze protein (AFP) variant, RD3, from, antarctic eel pout (Rhigophila dearborni) with enhanced activity has been, determined for the first time by nuclear magnetic resonance spectroscopy., RD3 comprises a unique translational topology of two homologous type III, AFP globular domains, each containing one flat, ice binding plane. The ice, binding plane of the N domain is located approximately 3.5 A "behind" that, of the C domain. The two ice binding planes are located laterally with an, angle of 32 +/- 12 degrees between the planes. These results suggest that, the C domain plane of RD3 binds first to the ice [1010] prism plane in the, &lt;0001&gt; direction, which induces successive ice binding of the N domain in, the &lt;0101&gt; direction. This manner of ice binding caused by the unique, structural topology of RD3 is thought to be crucial for the significant, enhancement of antifreeze activity, especially at low AFP concentrations.
 ==About this Structure==
-C89 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pachycara_brachycephalum Pachycara brachycephalum]]. Structure known Active Sites: IC1 and IC2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C89 OCA]].
+C89 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pachycara_brachycephalum Pachycara brachycephalum]. Structure known Active Sites: IC1 and IC2. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C89 OCA].
 ==Reference==
@@ Line 20: / Line 20: @@
 [[Category: thermal hysteresis protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:58:31 2007''
+''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 15:25:41 2007''