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| '''Exportins''' (XPO) or '''chromosomal maintenance protein''' mediate the nuclear export of cellular proteins with a leucine-rich export signal (NES) and RNA. XPO is regulated by GTPaseRan. RanGTP bind to XPO forming a ternary complex with the exported protein. The complex diffuses through the nuclear core complex to the cytoplasm and then dissociates. '''XPO-t''' exports tRNA from the nucleus<ref>PMID:17786152</ref>. | | '''Exportins''' (XPO) or '''chromosomal maintenance protein''' mediate the nuclear export of cellular proteins with a leucine-rich export signal (NES) and RNA. XPO is regulated by GTPaseRan. RanGTP bind to XPO forming a ternary complex with the exported protein. The complex diffuses through the nuclear core complex to the cytoplasm and then dissociates. '''XPO-t''' exports tRNA from the nucleus<ref>PMID:17786152</ref>. |
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| ==Structural highlighta == | | ==Structural highlights == |
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| <scene name='59/592125/Cv/2'>Yeast exportin-5 with GTPaseRan, Mg+2 and RNA</scene> (PDB code [[3icq]]). | | <scene name='59/592125/Cv/2'>Yeast exportin-5 with GTPaseRan, Mg+2 and RNA</scene>. |
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| <scene name='59/592125/Cv/6'>GTP binding site</scene> | | <scene name='59/592125/Cv/6'>GTP binding site</scene>. |
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| | <scene name='59/592125/Cv/7'>Mg coordination site</scene> (PDB code [[3icq]]).<ref>PMID:19680239</ref> |
| </StructureSection> | | </StructureSection> |
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Revision as of 10:22, 15 February 2016
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Exportins (XPO) or chromosomal maintenance protein mediate the nuclear export of cellular proteins with a leucine-rich export signal (NES) and RNA. XPO is regulated by GTPaseRan. RanGTP bind to XPO forming a ternary complex with the exported protein. The complex diffuses through the nuclear core complex to the cytoplasm and then dissociates. XPO-t exports tRNA from the nucleus[1].
Structural highlights
Yeast exportin-5 with GTPaseRan, Mg+2 and RNA.
GTP binding site.
Mg coordination site (PDB code 3icq).[2]
- ↑ Kohler A, Hurt E. Exporting RNA from the nucleus to the cytoplasm. Nat Rev Mol Cell Biol. 2007 Oct;8(10):761-73. PMID:17786152 doi:https://dx.doi.org/10.1038/nrm2255
- ↑ Cook AG, Fukuhara N, Jinek M, Conti E. Structures of the tRNA export factor in the nuclear and cytosolic states. Nature. 2009 Sep 3;461(7260):60-5. PMID:19680239 doi:10.1038/nature08394
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3D structures of exportin
Updated on 15-February-2016
{"openlevels":0}
- XPO-1
- 3icq, 4bsn – hXPO – human
- 1w9c – hXPO C terminal 6 heat repeats
- 3vyc – yXPO (mutant) - yeast
- 4fgv – XPO – Chaetomium thermophilum
- XPO-1 complexes
- 3gb8 – hXPO + RNA U transporter 1
- 3gjx – hXPO + RNA U transporter 1 + GTPaseRan
- 3m1i, 4gpt, 4gmx, 4hat, 4hb2 – yXPO (mutant) + GTP-binding nuclear protein + Ran-specific GTPase-activating protein
- 3wyf – yXPO + GTP-binding nuclear protein + Ran-specific GTPase-activating protein
- 3wyg – yXPO + GTP-binding nuclear protein + PKI-alpha
- 4hau, 4hav, 4haw, 4hax, 4hay, 4haz, 4hb0, 4hb3, 4hb4 – yXPO (mutant) + GTP-binding nuclear protein + Ran-specific GTPase-activating protein + antibiotic
- 3nby, 3nbz, 3nc0 – mXPO + RNA U transporter 1 + GTPaseRan - mouse
- 3nc1 – mXPO + GTPaseRan
- 2l1l – hXPO residues 504-630 + cAMP-dependent protein kinase inhibitor α - NMR
- XPO-t
- 3ibv – fyXPO – fission yeast
- 3icq – fyXPO + GTP-binding nuclear protein + RNA
- XPO-5
- 3a6p – hXPO + peptide + pre-microRNA
References
