4y0w

YeaZ from Pseudomonas aeruginosa

spinqualitylabelsall models 4y0w, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4y0w is a 5 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Publication Abstract from PubMed The Pseudomonas aeruginosa PA3685 locus encodes a conserved protein that shares 49% sequence identity with Escherichiacoli YeaZ, which was recently reported as involved in the biosynthesis of threonylcarbamoyl adenosine (t6A), a universal modified tRNA nucleoside. Many YeaZ orthologues were reported as "essential for life" among various bacterial species, suggesting a critical role for both these proteins and for the t6A biosynthetic pathway. We provide here evidences that PA3685 protein (PaYeaZ) is essential. Additionally, we describe its purification, crystallization, and crystallographic structure. The crystal structure shows that PaYeaZ is composed of two domains one of which is the platform to form protein-protein interaction involved either in homodimeric assembly or in the formation of the multiprotein complex required for the synthesis of t6A. These features make the PaYeaZ protein a potential target candidate for the design of novel inhibitors able to hinder the complex formation and expected to abolish the crucial activity of t6A synthesis. Crystal structure of YeaZ from Pseudomonas aeruginosa.,Vecchietti D, Ferrara S, Rusmini R, Macchi R, Milani M, Bertoni G Biochem Biophys Res Commun. 2016 Jan 6. pii: S0006-291X(16)30008-0. doi:, 10.1016/j.bbrc.2016.01.008. PMID:26768361 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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