Exonuclease: Difference between revisions
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'''Exonucleases''' (ExN) cleave nucleotides at the end of a polynucleotide chain<ref>PMID:14770007</ref>. In ''E. coli'' there are ExN I to VIII. ExN were found in human and yeast as well. | '''Exonucleases''' (ExN) cleave nucleotides at the end of a polynucleotide chain<ref>PMID:14770007</ref>. In ''E. coli'' there are ExN I to VIII. ExN were found in human and yeast as well. | ||
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* '''ExN-I''' cleaves single-stranded DNA.<br /> | * '''ExN-I''' cleaves single-stranded DNA.<br /> | ||
* '''ExN-II''' is associated with DNA polymerase.<br /> | * '''ExN-II''' is associated with DNA polymerase.<br /> | ||
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* '''ExN-V''' cleaves linear double-stranded DNA.<br /> | * '''ExN-V''' cleaves linear double-stranded DNA.<br /> | ||
* '''ExN-VIII''' requires a free 5’ OH group for reacting.<br /> | * '''ExN-VIII''' requires a free 5’ OH group for reacting.<br /> | ||
* For '''phage ExN''' see [[Sand box 211]]. | |||
<scene name='46/466467/Cv/2'>Mg coordination site</scene> in ''E. coli'' exonuclease I (PDB code [[1fxx]]).<ref>PMID:11101894</ref> Water molecules shown as red spheres. | <scene name='46/466467/Cv/2'>Mg coordination site</scene> in ''E. coli'' exonuclease I (PDB code [[1fxx]]).<ref>PMID:11101894</ref> Water molecules shown as red spheres. | ||
