2bra: Difference between revisions
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==Overview== | ==Overview== | ||
During development, neurons are guided to their targets by short- and, long-range attractive and repulsive cues. MICAL, a large multidomain, protein, is required for the combined action of semaphorins and plexins in, axon guidance. Here, we present the structure of the N-terminal region of, MICAL (MICAL(fd)) determined by x-ray diffraction to 2.0 A resolution. The, structure shows that MICAL(fd) is an FAD-containing module structurally, similar to aromatic hydroxylases and amine oxidases. In addition, we, present biochemical data that show that MICAL(fd) is a flavoenzyme that in, the presence of NADPH reduces molecular oxygen to H(2)O(2) (K(m,NAPDH) =, 222 microM; k(cat) = 77 sec(-1)), a molecule with known signaling, properties. We propose that the H(2)O(2) produced by this reaction may . | During development, neurons are guided to their targets by short- and, long-range attractive and repulsive cues. MICAL, a large multidomain, protein, is required for the combined action of semaphorins and plexins in, axon guidance. Here, we present the structure of the N-terminal region of, MICAL (MICAL(fd)) determined by x-ray diffraction to 2.0 A resolution. The, structure shows that MICAL(fd) is an FAD-containing module structurally, similar to aromatic hydroxylases and amine oxidases. In addition, we, present biochemical data that show that MICAL(fd) is a flavoenzyme that in, the presence of NADPH reduces molecular oxygen to H(2)O(2) (K(m,NAPDH) =, 222 microM; k(cat) = 77 sec(-1)), a molecule with known signaling, properties. We propose that the H(2)O(2) produced by this reaction may be, one of the signaling molecules involved in axon guidance by MICAL. | ||
==About this Structure== | ==About this Structure== | ||
2BRA is a | 2BRA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with CL and FAD as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BRA OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:27:44 2007'' | ||
