5ehk: Difference between revisions

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'''Unreleased structure'''


The entry 5ehk is ON HOLD  until Paper Publication
==Crystal structure of tRNA dependent lantibiotic dehydratase MibB from Microbispora sp. 107891==
<StructureSection load='5ehk' size='340' side='right' caption='[[5ehk]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ehk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EHK FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ehk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ehk OCA], [http://pdbe.org/5ehk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ehk RCSB], [http://www.ebi.ac.uk/pdbsum/5ehk PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Class I lantibiotic dehydratases dehydrate selected Ser/Thr residues of a precursor peptide. Recent studies demonstrated the requirement of glutamyl-tRNAGlu for Ser/Thr activation by one of these enzymes (NisB) from the Firmicute Lactococcus lactis. However, the generality of glutamyl-tRNAGlu usage and the tRNA specificity of lantibiotic dehydratases have not been established. Here we report the 2.7-A resolution crystal structure, along with the glutamyl-tRNAGlu utilization of MibB, a lantibiotic dehydratase from the Actinobacterium Microbispora sp. 107891 involved in the biosynthesis of the clinical candidate NAI-107. Biochemical assays revealed nucleotides A73 and U72 within the tRNAGlu acceptor stem to be important for MibB glutamyl-tRNAGlu usage. Using this knowledge, an expression system for the production of NAI-107 analogs in Escherichia coli was developed, overcoming the inability of MibB to utilize E. coli tRNAGlu. Our work provides evidence for a common tRNAGlu-dependent dehydration mechanism, paving the way for the characterization of lantibiotics from various phyla.


Authors: Hao, Y., Nair, S.K.
Structure and tRNA Specificity of MibB, a Lantibiotic Dehydratase from Actinobacteria Involved in NAI-107 Biosynthesis.,Ortega MA, Hao Y, Walker MC, Donadio S, Sosio M, Nair SK, van der Donk WA Cell Chem Biol. 2016 Feb 8. pii: S2451-9456(16)30001-0. doi:, 10.1016/j.chembiol.2015.11.017. PMID:26877024<ref>PMID:26877024</ref>


Description: Crystal structure of tRNA dependent lantibiotic dehydratase MibB from Microbispora sp. 107891
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Nair, S.K]]
<div class="pdbe-citations 5ehk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Hao, Y]]
[[Category: Hao, Y]]
[[Category: Nair, S K]]
[[Category: Hydrolase]]
[[Category: Lantibiotic dehydratase]]
[[Category: Microbispora sp. 107891]]
[[Category: Nai-107]]
[[Category: Trna dependent]]
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