Sandbox Reserved 1176: Difference between revisions

Line 28:

===Active State===

After determining the original structural state, <scene name='72/721547/Ntsr1-gw5/8'>NTSR1-GW5</scene>, as only active-like, the structure of NTSR1 was determined in ~~a fully~~ active state.<ref name="SPGP"/> By reverting back three of the original six mutations from the active-like structure on the basis of their location, NTSR1 gained near wild-type activity.<ref name="SPGP"/> The three reversions were Asp166, Leu310, and Phe358, and this protein was named <scene name='72/721548/Ntsr1-elf/6'>NTSR1-ELF</scene>. The revival of activity in NTSR1 indicated that the reverted amino acid residues (Asp166, Leu310, and Phe358) play significant roles in G-protein activity.<ref name="SPGP"/>

After determining the original structural state, <scene name='72/721547/Ntsr1-gw5/8'>NTSR1-GW5</scene>, as only active-like, the structure of NTSR1 was determined in an active state.<ref name="SPGP"/> By reverting back three of the original six mutations from the active-like structure on the basis of their location, NTSR1 gained near wild-type activity.<ref name="SPGP"/> The three reversions were Asp166, Leu310, and Phe358, and this protein was named <scene name='72/721548/Ntsr1-elf/6'>NTSR1-ELF</scene>. The revival of activity in NTSR1 indicated that the reverted amino acid residues (Asp166, Leu310, and Phe358) play significant roles in G-protein activity.<ref name="SPGP"/>

====Leu310====

Sandbox Reserved 1176: Difference between revisions

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 28: / Line 28: @@
 ===Active State===
-After determining the original structural state, <scene name='72/721547/Ntsr1-gw5/8'>NTSR1-GW5</scene>, as only active-like, the structure of NTSR1 was determined in a fully active state.<ref name="SPGP"/> By reverting back three of the original six mutations from the active-like structure on the basis of their location, NTSR1 gained near wild-type activity.<ref name="SPGP"/> The three reversions were Asp166, Leu310, and Phe358, and this protein was named <scene name='72/721548/Ntsr1-elf/6'>NTSR1-ELF</scene>. The revival of activity in NTSR1 indicated that the reverted amino acid residues (Asp166, Leu310, and Phe358) play significant roles in G-protein activity.<ref name="SPGP"/>
+After determining the original structural state, <scene name='72/721547/Ntsr1-gw5/8'>NTSR1-GW5</scene>, as only active-like, the structure of NTSR1 was determined in an active state.<ref name="SPGP"/> By reverting back three of the original six mutations from the active-like structure on the basis of their location, NTSR1 gained near wild-type activity.<ref name="SPGP"/> The three reversions were Asp166, Leu310, and Phe358, and this protein was named <scene name='72/721548/Ntsr1-elf/6'>NTSR1-ELF</scene>. The revival of activity in NTSR1 indicated that the reverted amino acid residues (Asp166, Leu310, and Phe358) play significant roles in G-protein activity.<ref name="SPGP"/>
 ====Leu310====