Sandbox Reserved 1176: Difference between revisions
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Danny Cotter (talk | contribs) No edit summary |
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On the extracellular side of the protein is the | On the extracellular side of the protein is the | ||
<scene name='72/721547/Hydrophobic_binding_pocket/5'>hydrophobic binding pocket</scene>. <ref name="SONT"/> | <scene name='72/721547/Hydrophobic_binding_pocket/5'>hydrophobic binding pocket</scene>. <ref name="SONT"/> | ||
One key residue in this pocket is a Phenylalanine at position 358, which takes part in a network of hydrophobic stacking interactions | One key residue in this pocket is a Phenylalanine at position 358, which takes part in a network of hydrophobic stacking interactions<ref name="SPGP"/>. These interactions stabilize the Trp321 and Tyr324 residues allowing Tyr324 to interact with the '''[https://en.wikipedia.org/wiki/C-terminus C-terminal]''' | ||
<scene name='72/721547/Hydrophobic_binding_pocket/6'>Leu13 residue of the NTS ligand</scene> | <scene name='72/721547/Hydrophobic_binding_pocket/6'>Leu13 residue of the NTS ligand</scene> | ||
via '''[https://en.wikipedia.org/wiki/Van_der_Waals_force Van der Waals interactions]''' .<ref name="SONT"/><ref name="SPGP"/> | via '''[https://en.wikipedia.org/wiki/Van_der_Waals_force Van der Waals interactions]''' .<ref name="SONT"/><ref name="SPGP"/> | ||
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==Activation of NTSR1== | ==Activation of NTSR1== | ||
Since wild type NTSR1 was unstable in detergent solution for imaging, six residues in the protein were mutated for stabilization. | Since wild type NTSR1 was unstable in detergent solution for imaging, six residues in the protein were mutated for stabilization.<ref name="SONT"/> <ref name="SPGP"/> | ||
===Active-Like State=== | ===Active-Like State=== | ||
