5b2h: Difference between revisions
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==Crystal structure of HA33 from Clostridium botulinum serotype C strain Yoichi== | |||
<StructureSection load='5b2h' size='340' side='right' caption='[[5b2h]], [[Resolution|resolution]] 2.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5b2h]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B2H FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b2h OCA], [http://pdbe.org/5b2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b2h RCSB], [http://www.ebi.ac.uk/pdbsum/5b2h PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Clostridium botulinum produces a large toxin complex (L-TC) comprising botulinum neurotoxin associated with auxiliary nontoxic proteins. A complex of 33- and 17-kDa hemagglutinins (an HA-33/HA-17 trimer) enhances L-TC transport across the intestinal epithelial cell layer via binding HA-33 to a sugar on the cell surface. At least two subtypes of serotype C/D HA-33 exhibit differing preferences for the sugars sialic acid and galactose. Here, we compared the three-dimensional structures of the galactose-binding HA-33 and HA-33/HA-17 trimers produced by the C-Yoichi strain. Comparisons of serotype C/D HA-33 sequences reveal a variable region with relatively low sequence similarity across the C. botulinum strains; the variability of this region may influence the manner of sugar-recognition by HA-33. Crystal structures of sialic acid- and galactose-binding HA-33 are broadly similar in appearance. However, small-angle X-ray scattering revealed distinct solution structures for HA-33/HA-17 trimers. A structural change in the C-terminal variable region of HA-33 might cause a dramatic shift in the conformation and sugar-recognition mode of HA-33/HA-17 trimer. | |||
Conformational divergence in the HA-33/HA-17 trimer of serotype C and D botulinum toxin complex.,Sagane Y, Hayashi S, Akiyama T, Matsumoto T, Hasegawa K, Yamano A, Suzuki T, Niwa K, Watanabe T, Yajima S Biochem Biophys Res Commun. 2016 May 27. pii: S0006-291X(16)30821-X. doi:, 10.1016/j.bbrc.2016.05.113. PMID:27237978<ref>PMID:27237978</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5b2h" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Akiyama, T]] | |||
[[Category: Hasegawa, K]] | |||
[[Category: Hayashi, S]] | |||
[[Category: Matsumoto, T]] | |||
[[Category: Niwa, K]] | [[Category: Niwa, K]] | ||
[[Category: Sagane, Y]] | [[Category: Sagane, Y]] | ||
[[Category: Suzuki, T]] | [[Category: Suzuki, T]] | ||
[[Category: Watanabe, T]] | [[Category: Watanabe, T]] | ||
[[Category: Yajima, S]] | |||
[[Category: Yamano, A]] | |||
[[Category: Hemagglutinin]] | |||
[[Category: Sugar binding protein]] | |||
Revision as of 15:09, 20 June 2016
Crystal structure of HA33 from Clostridium botulinum serotype C strain Yoichi
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