5ffs: Difference between revisions

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'''Unreleased structure'''


The entry 5ffs is ON HOLD  until Paper Publication
==Crystal Structure of Surfactant Protein-A Y164A Mutant==
<StructureSection load='5ffs' size='340' side='right' caption='[[5ffs]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ffs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FFS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SME:METHIONINE+SULFOXIDE'>SME</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ffr|5ffr]], [[5fft|5fft]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ffs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ffs OCA], [http://pdbe.org/5ffs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ffs RCSB], [http://www.ebi.ac.uk/pdbsum/5ffs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ffs ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/SFTPA_RAT SFTPA_RAT]] In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Surfactant protein A (SP-A) is a collagenous C-type lectin (collectin) that is critical for pulmonary defense against inhaled microorganisms. Bifunctional avidity of SP-A for pathogen-associated molecular patterns (PAMPs) such as lipid A and for dipalmitoylphosphatidylcholine (DPPC), the major component of surfactant membranes lining the air-liquid interface of the lung, ensures that the protein is poised for first-line interactions with inhaled pathogens. To improve our understanding of the motifs that are required for interactions with microbes and surfactant structures, we explored the role of the tyrosine-rich binding surface on the carbohydrate recognition domain of SP-A in the interaction with DPPC and lipid A using crystallography, site-directed mutagenesis, and molecular dynamics simulations. Critical binding features for DPPC binding include a three-walled tyrosine cage that binds the choline headgroup through cation-pi interactions and a positively charged cluster that binds the phosphoryl group. This basic cluster is also critical for binding of lipid A, a bacterial PAMP and target for SP-A. Molecular dynamics simulations further predict that SP-A binds lipid A more tightly than DPPC. These results suggest that the differential binding properties of SP-A favor transfer of the protein from surfactant DPPC to pathogen membranes containing appropriate lipid PAMPs to effect key host defense functions.


Authors: Goh, B.C., Wu, H., Rynkiewicz, M.J., Schulten, K., Seaton, B.A., McCormack, F.X.
Elucidation of Lipid Binding Sites on Lung Surfactant Protein A Using X-ray Crystallography, Mutagenesis, and Molecular Dynamics Simulations.,Goh BC, Wu H, Rynkiewicz MJ, Schulten K, Seaton BA, McCormack FX Biochemistry. 2016 Jul 5;55(26):3692-701. doi: 10.1021/acs.biochem.6b00048. Epub , 2016 Jun 21. PMID:27324153<ref>PMID:27324153</ref>


Description: Crystal Structure of Surfactant Protein-A Y164A Mutant
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Mccormack, F.X]]
<div class="pdbe-citations 5ffs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Goh, B C]]
[[Category: McCormack, F X]]
[[Category: Rynkiewicz, M J]]
[[Category: Schulten, K]]
[[Category: Seaton, B A]]
[[Category: Wu, H]]
[[Category: Wu, H]]
[[Category: Rynkiewicz, M.J]]
[[Category: Carbohydrate binding]]
[[Category: Seaton, B.A]]
[[Category: Collectin]]
[[Category: Goh, B.C]]
[[Category: Lectin]]
[[Category: Schulten, K]]
[[Category: Lipid binding]]
[[Category: Sugar binding protein]]
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