5jdb: Difference between revisions
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==Binding specificity of P[8] VP8* proteins of rotavirus vaccine strains with histo-blood group antigens== | |||
<StructureSection load='5jdb' size='340' side='right' caption='[[5jdb]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5jdb]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JDB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JDB FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jdb OCA], [http://pdbe.org/5jdb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jdb RCSB], [http://www.ebi.ac.uk/pdbsum/5jdb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jdb ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/E2EA82_9REOV E2EA82_9REOV]] Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment.[SAAS:SAAS00136874] VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact.[SAAS:SAAS00136880] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
RotaTeq((R)) and Rotarix are two common human rotavirus (RV) vaccines currently on the market worldwide. Recent studies indicate histo-blood group antigens (HBGAs) may be attachment factors for RVs. The P[8] VP8* proteins of RotaTeq and Rotarix were expressed and purified, and their binding specificities were evaluated. Saliva-based binding assays showed that the VP8* proteins bound to the saliva samples of secretors irrespective of ABO blood types. However, in the oligosaccharide binding assay, the VP8* proteins displayed no specific binding to the HBGAs tested, including Lewis b and H1. The structure of RotaTeq P[8] VP8* was solved at 1.9A. Structural comparisons revealed that the putative receptor binding site was different to that of other genotypes and displayed a novel potential binding region. These findings indicate RotaTeq and Rotarix may have better efficiency in areas with a high percentage of secretors. | |||
Binding specificity of P[8] VP8* proteins of rotavirus vaccine strains with histo-blood group antigens.,Sun X, Guo N, Li D, Jin M, Zhou Y, Xie G, Pang L, Zhang Q, Cao Y, Duan ZJ Virology. 2016 Aug;495:129-35. doi: 10.1016/j.virol.2016.05.010. Epub 2016 May, 19. PMID:27209447<ref>PMID:27209447</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5jdb" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Cao, Y]] | |||
[[Category: Duan, Z]] | |||
[[Category: Guo, N]] | |||
[[Category: Jin, M]] | |||
[[Category: Li, D]] | |||
[[Category: Pang, L]] | |||
[[Category: Sun, X]] | |||
[[Category: Xie, G]] | |||
[[Category: Zhang, Q]] | |||
[[Category: Zhou, Y]] | |||
[[Category: Rotavirus]] | |||
[[Category: Vaccine]] | |||
[[Category: Viral protein]] | |||
[[Category: Vp8]] | |||
