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The | ==The high resolution structure of a novel alpha-L-arabinofuranosidase (CtGH43) from Clostridium thermocellum ATCC 27405== | ||
<StructureSection load='5a8d' size='340' side='right' caption='[[5a8d]], [[Resolution|resolution]] 1.65Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5a8d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A8D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A8D FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a8c|5a8c]]</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-reducing_end_alpha-L-arabinofuranosidase Non-reducing end alpha-L-arabinofuranosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.55 3.2.1.55] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a8d OCA], [http://pdbe.org/5a8d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a8d RCSB], [http://www.ebi.ac.uk/pdbsum/5a8d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a8d ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The truncated carbohydrate-active enzyme belonging to family 43 glycoside hydrolase from Clostridium thermocellum (CtGH43) is an alpha-L-arabinofuranosidase that in combination with endoxylanase leads to complete breakdown of L-arabinosyl-substituted xylans. The recombinant enzyme CtGH43 from C. thermocellum was overexpressed in Escherichia coli and purified by immobilized metal-ion affinity chromatography. The recombinant CtGH43 has a molecular mass of 35.86 kDa. Preliminary structural characterization was carried out on CtGH43 crystallized from different conditions, which gave either cube-shaped or brick-shaped crystals. These diffracted to a resolution of 1.65 A for the cubic form and 1.1 A for the monoclinic form. Molecular replacement was used to solve the CtGH43 structure. | |||
Crystallization and preliminary X-ray crystallographic analysis of a novel alpha-L-arabinofuranosidase (CtGH43) from Clostridium thermocellum ATCC 27405.,Goyal A, Ahmed S, Fontes CM, Najmudin S Acta Crystallogr F Struct Biol Commun. 2014 May;70(Pt 5):616-8. doi:, 10.1107/S2053230X14006402. Epub 2014 Apr 15. PMID:24817722<ref>PMID:24817722</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5a8d" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Non-reducing end alpha-L-arabinofuranosidase]] | |||
[[Category: Ahmed, S]] | |||
[[Category: Fontes, C M.G A]] | |||
[[Category: Goyal, A]] | [[Category: Goyal, A]] | ||
[[Category: Najmudin, S]] | |||
[[Category: Sharma, K]] | [[Category: Sharma, K]] | ||
[[Category: | [[Category: 5-fold-beta-propeller]] | ||
[[Category: | [[Category: Alpha-l-arabinofuranosidase]] | ||
[[Category: | [[Category: C. thermocellum]] | ||
[[Category: Ctgh43]] | |||
[[Category: Glycoside hydrolase]] | |||
[[Category: Hydrolase]] | |||
Revision as of 15:33, 27 July 2016
The high resolution structure of a novel alpha-L-arabinofuranosidase (CtGH43) from Clostridium thermocellum ATCC 27405
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