3ml3: Difference between revisions

Revision as of 14:15, 4 August 2016

Crystal structure of the IcsA autochaperone region

Structural highlights

3ml3 is a 1 chain structure with sequence from "shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	CP0182, icsA, virG ("Shigella paradysenteriae" Weldin 1927)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ICSA_SHIFL] Essential for bacterial spreading by eliciting polar deposition of filamentous actin (actin-based motility). Inside the host cell mediates nucleation and polymerization of actin molecules on the bacterial surface, which provides the propulsive force for intracellular movement and intercellular dissemination of the bacterium. During invasion of mammalian cells, triggers autophagy by binding to APG5L. Interaction with IcsB leads to escape from the autophagic host defense system. Also binds ATP and displays weak ATPase activity.^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

The IcsA (intracellular spread gene A) autotransporter from Shigella flexneri is a key virulence factor. We identified a stable fragment comprising residues 591 to 758, which corresponds to the autochaperone region of the IcsA passenger domain. We showed that thermal unfolding of the autochaperone region is reversible and determined its crystal structure at 2.0-A resolution.

Crystal Structure of the Autochaperone Region from the Shigella flexneri Autotransporter IcsA.,Kuhnel K, Diezmann D J Bacteriol. 2011 Apr;193(8):2042-5. Epub 2011 Feb 18. PMID:21335457^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fukuda I, Suzuki T, Munakata H, Hayashi N, Katayama E, Yoshikawa M, Sasakawa C. Cleavage of Shigella surface protein VirG occurs at a specific site, but the secretion is not essential for intracellular spreading. J Bacteriol. 1995 Apr;177(7):1719-26. PMID:7896693
↑ Bernardini ML, Mounier J, d'Hauteville H, Coquis-Rondon M, Sansonetti PJ. Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin. Proc Natl Acad Sci U S A. 1989 May;86(10):3867-71. PMID:2542950
↑ d'Hauteville H, Sansonetti PJ. Phosphorylation of IcsA by cAMP-dependent protein kinase and its effect on intracellular spread of Shigella flexneri. Mol Microbiol. 1992 Apr;6(7):833-41. PMID:1602963
↑ Suzuki T, Miki H, Takenawa T, Sasakawa C. Neural Wiskott-Aldrich syndrome protein is implicated in the actin-based motility of Shigella flexneri. EMBO J. 1998 May 15;17(10):2767-76. PMID:9582270 doi:10.1093/emboj/17.10.2767
↑ Ogawa M, Yoshimori T, Suzuki T, Sagara H, Mizushima N, Sasakawa C. Escape of intracellular Shigella from autophagy. Science. 2005 Feb 4;307(5710):727-31. Epub 2004 Dec 2. PMID:15576571 doi:10.1126/science.1106036
↑ Kuhnel K, Diezmann D. Crystal Structure of the Autochaperone Region from the Shigella flexneri Autotransporter IcsA. J Bacteriol. 2011 Apr;193(8):2042-5. Epub 2011 Feb 18. PMID:21335457 doi:10.1128/JB.00790-10

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OCA

[1] Fukuda I, Suzuki T, Munakata H, Hayashi N, Katayama E, Yoshikawa M, Sasakawa C. Cleavage of Shigella surface protein VirG occurs at a specific site, but the secretion is not essential for intracellular spreading. J Bacteriol. 1995 Apr;177(7):1719-26. PMID:7896693

[2] Bernardini ML, Mounier J, d'Hauteville H, Coquis-Rondon M, Sansonetti PJ. Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin. Proc Natl Acad Sci U S A. 1989 May;86(10):3867-71. PMID:2542950

[3] 'Hauteville H, Sansonetti PJ. Phosphorylation of IcsA by cAMP-dependent protein kinase and its effect on intracellular spread of Shigella flexneri. Mol Microbiol. 1992 Apr;6(7):833-41. PMID:1602963

[4] Suzuki T, Miki H, Takenawa T, Sasakawa C. Neural Wiskott-Aldrich syndrome protein is implicated in the actin-based motility of Shigella flexneri. EMBO J. 1998 May 15;17(10):2767-76. PMID:9582270 doi:10.1093/emboj/17.10.2767

[5] Ogawa M, Yoshimori T, Suzuki T, Sagara H, Mizushima N, Sasakawa C. Escape of intracellular Shigella from autophagy. Science. 2005 Feb 4;307(5710):727-31. Epub 2004 Dec 2. PMID:15576571 doi:10.1126/science.1106036

[6] Kuhnel K, Diezmann D. Crystal Structure of the Autochaperone Region from the Shigella flexneri Autotransporter IcsA. J Bacteriol. 2011 Apr;193(8):2042-5. Epub 2011 Feb 18. PMID:21335457 doi:10.1128/JB.00790-10

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@@ Line 1: / Line 1: @@
 ==Crystal structure of the IcsA autochaperone region==
 <StructureSection load='3ml3' size='340' side='right' caption='[[3ml3]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ml3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ML3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ML3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ml3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ML3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ML3 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CP0182, icsA, virG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 Shigella flexneri])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CP0182, icsA, virG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ml3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ml3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ml3 RCSB], [http://www.ebi.ac.uk/pdbsum/3ml3 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ml3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ml3 OCA], [http://pdbe.org/3ml3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ml3 RCSB], [http://www.ebi.ac.uk/pdbsum/3ml3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ml3 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3ml3" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Shigella flexneri]]
+[[Category: Shigella paradysenteriae weldin 1927]]
 [[Category: Diezmann, D]]
 [[Category: Kuhnel, K]]

3ml3: Difference between revisions

Revision as of 14:15, 4 August 2016

Crystal structure of the IcsA autochaperone region

Structural highlights

Function

Publication Abstract from PubMed

References

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