4bxs

Crystal Structure of the Prothrombinase Complex from the Venom of Pseudonaja Textilis

spinqualitylabelsall models 4bxs, resolution 3.32Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4bxs is a 2 chain structure with sequence from Eastern brown snake. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BMA, CA, CU, NAG, FUC, MAN Related: 4bxw Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [FA5V_PSETE] Snake prothrombin activator that attacks the hemostatic system of prey. This non-catalytic subunit is functionally similar to blood coagulation factor V. It serves as a critical cofactor for the prothrombinase activity of the catalytic subunit, which is similar to the blood coagulation factor X. Publication Abstract from PubMed The prothrombinase complex, composed of the protease factor (f)Xa and cofactor fVa, efficiently converts prothrombin to thrombin by specific, sequential cleavage at two sites. How the complex assembles and its mechanism of prothrombin processing is of central importance to human health and disease, since insufficient thrombin generation is the root-cause of hemophilia and excessive thrombin production results in thrombosis. Efforts to determine the crystal structure of the prothrombinase complex have been thwarted by the dependence of complex formation on phospholipid membrane association. Pseutarin C is an intrinsically stable prothrombinase complex preassembled in the venom gland of the Australian Eastern Brown Snake (Pseudonaja textilis). Here we report the crystal structures of the fX-fV complex and of activated fXa from P. textilis venom, and the derived model of active Pseutarin C. Structural analysis supports a single substrate binding channel on fVa, to which prothrombin and the intermediate meizothrombin bind in two different orientations, providing insight into the architecture and mechanism of the prothrombinase complex- the molecular engine of blood coagulation. Crystal structure of the prothrombinase complex from the venom of Pseudonaja textilis.,Lechtenberg BC, Murray-Rust TA, Johnson DJ, Adams TE, Krishnaswamy S, Camire RM, Huntington JA Blood. 2013 Jul 18. PMID:23869089 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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