4hpq: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal Structure of the Atg17-Atg31-Atg29 Complex== | |||
<StructureSection load='4hpq' size='340' side='right' caption='[[4hpq]], [[Resolution|resolution]] 3.06Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4hpq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_thermotolerans Kluyveromyces thermotolerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HPQ FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Atg31, KLTH0C07942g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559295 Kluyveromyces thermotolerans]), Atg29, KLTH0D11660g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559295 Kluyveromyces thermotolerans]), Atg17, KLTH0D15642g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559295 Kluyveromyces thermotolerans])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpq OCA], [http://pdbe.org/4hpq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hpq RCSB], [http://www.ebi.ac.uk/pdbsum/4hpq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hpq ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Macroautophagy is a bulk clearance mechanism in which the double-membraned phagophore grows and engulfs cytosolic material. In yeast, the phagophore nucleates from a cluster of 20-30 nm diameter Atg9-containing vesicles located at a multiprotein assembly known as the preautophagosomal structure (PAS). The crystal structure of a 2:2:2 complex of the earliest acting PAS proteins, Atg17, Atg29, and Atg31, was solved at 3.05 A resolution. Atg17 is crescent shaped with a 10 nm radius of curvature. Dimerization of the Atg17-Atg31-Atg29 complex is critical for both PAS formation and autophagy, and each dimer contains two separate and complete crescents. Upon induction of autophagy, Atg17-Atg31-Atg29 assembles with Atg1 and Atg13, which in turn initiates the formation of the phagophore. The C-terminal EAT domain of Atg1 was shown to sense membrane curvature, dimerize, and tether lipid vesicles. These data suggest a structural mechanism for the organization of Atg9 vesicles into the early phagophore. | |||
Architecture of the atg17 complex as a scaffold for autophagosome biogenesis.,Ragusa MJ, Stanley RE, Hurley JH Cell. 2012 Dec 21;151(7):1501-12. doi: 10.1016/j.cell.2012.11.028. Epub 2012 Dec , 6. PMID:23219485<ref>PMID:23219485</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4hpq" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
[[ | *[[Autophagy-related protein|Autophagy-related protein]] | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Kluyveromyces thermotolerans]] | |||
[[Category: Hurley, J H]] | [[Category: Hurley, J H]] | ||
[[Category: Ragusa, M J]] | [[Category: Ragusa, M J]] | ||
Revision as of 22:33, 4 August 2016
Crystal Structure of the Atg17-Atg31-Atg29 Complex
| ||||||||||||
