4eob: Difference between revisions

Line 1:

~~{{STRUCTURE_4eob| PDB=4eob | SCENE= }}~~

~~===Structure of the type VI peptidoglycan amidase effector Tse1 from Pseudomonas aeruginosa===~~

~~{{ABSTRACT_PUBMED_22813741}}~~

==~~About this~~ Structure==

==Structure of the type VI peptidoglycan amidase effector Tse1 from Pseudomonas aeruginosa==

[[4eob]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EOB OCA].

[[~~Category~~: Pseudomonas aeruginosa ~~pao1~~]]

== Structural highlights ==

[[Category: Chou, S.]]

<table><tr><td colspan='2'>[[4eob]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EOB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EOB FirstGlance]. <br>

[[Category: Mougous, J D.]]

</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA1844 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eob OCA], [http://pdbe.org/4eob PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eob RCSB], [http://www.ebi.ac.uk/pdbsum/4eob PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eob ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

The target range of a bacterial secretion system can be defined by effector substrate specificity or by the efficacy of effector delivery. Here, we report the crystal structure of Tse1, a type VI secretion (T6S) bacteriolytic amidase effector from Pseudomonas aeruginosa. Consistent with its role as a toxin, Tse1 has a more accessible active site than related housekeeping enzymes. The activity of Tse1 against isolated peptidoglycan shows its capacity to act broadly against Gram-negative bacteria and even certain Gram-positive species. Studies with intact cells indicate that Gram-positive bacteria can remain vulnerable to Tse1 despite cell wall modifications. However, interbacterial competition studies demonstrate that Tse1-dependent lysis is restricted to Gram-negative targets. We propose that the previously observed specificity for T6S against Gram-negative bacteria is a consequence of high local effector concentration achieved by T6S-dependent targeting to its site of action rather than inherent effector substrate specificity.

Structure of a peptidoglycan amidase effector targeted to Gram-negative bacteria by the type VI secretion system.,Chou S, Bui NK, Russell AB, Lexa KW, Gardiner TE, LeRoux M, Vollmer W, Mougous JD Cell Rep. 2012 Jun 28;1(6):656-64. Epub 2012 May 31. PMID:22813741<ref>PMID:22813741</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Pseae]]

[[Category: Chou, S]]

[[Category: Mougous, J D]]

[[Category: Amidase]]

[[Category: Bacteriolytic]]

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-{{STRUCTURE_4eob|  PDB=4eob  |  SCENE=  }}
-===Structure of the type VI peptidoglycan amidase effector Tse1 from Pseudomonas aeruginosa===
-{{ABSTRACT_PUBMED_22813741}}
-==About this Structure==
+==Structure of the type VI peptidoglycan amidase effector Tse1 from Pseudomonas aeruginosa==
-[[4eob]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EOB OCA].
+<StructureSection load='4eob' size='340' side='right' caption='[[4eob]], [[Resolution|resolution]] 2.61&Aring;' scene=''>
-[[Category: Pseudomonas aeruginosa pao1]]
+== Structural highlights ==
-[[Category: Chou, S.]]
+<table><tr><td colspan='2'>[[4eob]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EOB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EOB FirstGlance]. <br>
-[[Category: Mougous, J D.]]
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA1844 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eob OCA], [http://pdbe.org/4eob PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eob RCSB], [http://www.ebi.ac.uk/pdbsum/4eob PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eob ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The target range of a bacterial secretion system can be defined by effector substrate specificity or by the efficacy of effector delivery. Here, we report the crystal structure of Tse1, a type VI secretion (T6S) bacteriolytic amidase effector from Pseudomonas aeruginosa. Consistent with its role as a toxin, Tse1 has a more accessible active site than related housekeeping enzymes. The activity of Tse1 against isolated peptidoglycan shows its capacity to act broadly against Gram-negative bacteria and even certain Gram-positive species. Studies with intact cells indicate that Gram-positive bacteria can remain vulnerable to Tse1 despite cell wall modifications. However, interbacterial competition studies demonstrate that Tse1-dependent lysis is restricted to Gram-negative targets. We propose that the previously observed specificity for T6S against Gram-negative bacteria is a consequence of high local effector concentration achieved by T6S-dependent targeting to its site of action rather than inherent effector substrate specificity.
+Structure of a peptidoglycan amidase effector targeted to Gram-negative bacteria by the type VI secretion system.,Chou S, Bui NK, Russell AB, Lexa KW, Gardiner TE, LeRoux M, Vollmer W, Mougous JD Cell Rep. 2012 Jun 28;1(6):656-64. Epub 2012 May 31. PMID:22813741<ref>PMID:22813741</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4eob" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Pseae]]
+[[Category: Chou, S]]
+[[Category: Mougous, J D]]
 [[Category: Amidase]]
 [[Category: Bacteriolytic]]