3qdc: Difference between revisions

Revision as of 06:18, 5 August 2016

Crystal structure of Natronomonas pharaonis sensory rhodopsin II in the active state

Structural highlights

3qdc is a 1 chain structure with sequence from Atcc 35678. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	BOG, L2P, LFA, RET
Gene:	sop2, sopII (ATCC 35678)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[BACS2_NATPH] Involved in the control of phototaxis. Seems to activate a methyl-accepting protein (HTR-II). Photoreceptor for blue light.

Publication Abstract from PubMed

The molecular mechanism of transmembrane signal transduction is still a pertinent question in cellular biology. Generally, a receptor can transfer an external signal via its cytoplasmic surface, as found for G-protein-coupled receptors such as rhodopsin, or via the membrane domain, such as that in sensory rhodopsin II (SRII) in complex with its transducer, HtrII. In the absence of HtrII, SRII functions as a proton pump. Here, we report on the crystal structure of the active state of uncomplexed SRII from Natronomonas pharaonis, NpSRII. The problem with a dramatic loss of diffraction quality upon loading of the active state was overcome by growing better crystals and by reducing the occupancy of the state. The conformational changes in the region comprising helices F and G are similar to those observed for the NpSRII-transducer complex but are much more pronounced. The meaning of these differences for the understanding of proton pumping and signal transduction by NpSRII is discussed.

Active State of Sensory Rhodopsin II: Structural Determinants for Signal Transfer and Proton Pumping.,Gushchin I, Reshetnyak A, Borshchevskiy V, Ishchenko A, Round E, Grudinin S, Engelhard M, Buldt G, Gordeliy V J Mol Biol. 2011 Aug 4. PMID:21840321^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gushchin I, Reshetnyak A, Borshchevskiy V, Ishchenko A, Round E, Grudinin S, Engelhard M, Buldt G, Gordeliy V. Active State of Sensory Rhodopsin II: Structural Determinants for Signal Transfer and Proton Pumping. J Mol Biol. 2011 Aug 4. PMID:21840321 doi:10.1016/j.jmb.2011.07.022

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OCA

[1] Gushchin I, Reshetnyak A, Borshchevskiy V, Ishchenko A, Round E, Grudinin S, Engelhard M, Buldt G, Gordeliy V. Active State of Sensory Rhodopsin II: Structural Determinants for Signal Transfer and Proton Pumping. J Mol Biol. 2011 Aug 4. PMID:21840321 doi:10.1016/j.jmb.2011.07.022

[1]

@@ Line 1: / Line 1: @@
 ==Crystal structure of Natronomonas pharaonis sensory rhodopsin II in the active state==
 <StructureSection load='3qdc' size='340' side='right' caption='[[3qdc]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3qdc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QDC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3qdc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35678 Atcc 35678]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QDC FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=L2P:2,3-DI-PHYTANYL-GLYCEROL'>L2P</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sop2, sopII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2257 Natronomonas pharaonis])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sop2, sopII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2257 ATCC 35678])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qdc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qdc RCSB], [http://www.ebi.ac.uk/pdbsum/3qdc PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qdc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdc OCA], [http://pdbe.org/3qdc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qdc RCSB], [http://www.ebi.ac.uk/pdbsum/3qdc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qdc ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3qdc" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 25: / Line 27: @@
 __TOC__
 </StructureSection>
-[[Category: Natronomonas pharaonis]]
+[[Category: Atcc 35678]]
 [[Category: Borshchevskiy, V]]
 [[Category: Buldt, G]]

3qdc: Difference between revisions

Revision as of 06:18, 5 August 2016

Crystal structure of Natronomonas pharaonis sensory rhodopsin II in the active state

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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