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{{STRUCTURE_3f8f|  PDB=3f8f  |  SCENE=  }}
===Crystal structure of multidrug binding transcriptional regulator LmrR complexed with Daunomycin===
{{ABSTRACT_PUBMED_19096365}}


==About this Structure==
==Crystal structure of multidrug binding transcriptional regulator LmrR complexed with Daunomycin==
[[3f8f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Laclm Laclm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F8F OCA].  
<StructureSection load='3f8f' size='340' side='right' caption='[[3f8f]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3f8f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Laclm Laclm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3F8F FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DM1:DAUNOMYCIN'>DM1</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3f8b|3f8b]], [[3f8c|3f8c]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lmrR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=416870 LACLM])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f8f OCA], [http://pdbe.org/3f8f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f8f RCSB], [http://www.ebi.ac.uk/pdbsum/3f8f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3f8f ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f8/3f8f_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3f8f ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
LmrR is a PadR-related transcriptional repressor that regulates the production of LmrCD, a major multidrug ABC transporter in Lactococcus lactis. Transcriptional regulation is presumed to follow a drug-sensitive induction mechanism involving the direct binding of transporter ligands to LmrR. Here, we present crystal structures of LmrR in an apo state and in two drug-bound states complexed with Hoechst 33342 and daunomycin. LmrR shows a common topology containing a typical beta-winged helix-turn-helix domain with an additional C-terminal helix involved in dimerization. Its dimeric organization is highly unusual with a flat-shaped hydrophobic pore at the dimer centre serving as a multidrug-binding site. The drugs bind in a similar manner with their aromatic rings sandwiched in between the indole groups of two dimer-related tryptophan residues. Multidrug recognition is facilitated by conformational plasticity and the absence of drug-specific hydrogen bonds. Combined analyses using site-directed mutagenesis, fluorescence-based drug binding and protein-DNA gel shift assays reveal an allosteric coupling between the multidrug- and DNA-binding sites of LmrR that most likely has a function in the induction mechanism.


==Reference==
Structure of the transcriptional regulator LmrR and its mechanism of multidrug recognition.,Madoori PK, Agustiandari H, Driessen AJ, Thunnissen AM EMBO J. 2008 Dec 18. PMID:19096365<ref>PMID:19096365</ref>
<ref group="xtra">PMID:019096365</ref><references group="xtra"/><references/>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3f8f" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Laclm]]
[[Category: Laclm]]
[[Category: Agustiandari, H.]]
[[Category: Agustiandari, H]]
[[Category: Driessen, A J.M.]]
[[Category: Driessen, A J.M]]
[[Category: Madoori, P K.]]
[[Category: Madoori, P K]]
[[Category: Thunnissen, A M.W H.]]
[[Category: Thunnissen, A M.W H]]
[[Category: Transcription regulator]]
[[Category: Transcription regulator]]
[[Category: Winged helix turn helix]]
[[Category: Winged helix turn helix]]
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