5iaz: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

The ~~entry~~ 5iaz is ~~ON HOLD until Paper~~ Publication

==The C-terminal domain of rice beta-galactosidase 1==

== Structural highlights ==

<table><tr><td colspan='2'>[[5iaz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IAZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IAZ FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iaz OCA], [http://pdbe.org/5iaz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iaz RCSB], [http://www.ebi.ac.uk/pdbsum/5iaz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iaz ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Most plant beta-galactosidases, which belong to glycoside hydrolase family 35, have a C-terminal domain homologous to animal galactose and rhamnose-binding lectins. To investigate the structure and function of this domain, the C-terminal domain of the rice (Oryza sativa L.) beta-galactosidase 1 (OsBGal1 Cter) was expressed in Escherichia coli and purified to homogeneity. The free OsBGal1 Cter is monomeric with a native molecular weight of 15kDa. NMR spectroscopy indicated that OsBGal1 Cter comprises five beta-strands and one alpha-helix. The structure of this domain is similar to lectin domains from animals, but loops A and C of OsBGal1 Cter are longer than the corresponding loops from related animal lectins with known structures. In addition, loop A of OsBGal1 Cter was not well defined, suggesting it is flexible. Although OsBGal1 Cter was predicted to be a galactose/rhamnose-binding domain, binding with rhamnose, galactose, glucose, beta-1,4-d-galactobiose and raffinose could not be observed in NMR experiments.

~~Authors:~~ Rimlumduan, T., Hua~~, Y.-l.~~, Tanaka, T., Ketudat-~~Cairns~~, J.R.

Structure of a plant beta-galactosidase C-terminal domain.,Rimlumduan T, Hua YL, Tanaka T, Ketudat Cairns JR Biochim Biophys Acta. 2016 Jul 22;1864(10):1411-1418. doi:, 10.1016/j.bbapap.2016.07.005. PMID:27451952<ref>PMID:27451952</ref>

~~Description: The C~~-~~terminal domain of rice beta~~-~~galactosidase 1~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

~~[[Category: Unreleased Structures]]~~

</div>

[[Category: Hua, Y~~.-L~~]]

[[Category: Ketudat-Cairns, J.R]]

== References ==

__TOC__

</StructureSection>

[[Category: Hua, Y l]]

[[Category: Ketudat-Cairns, J R]]

[[Category: Rimlumduan, T]]

[[Category: Tanaka, T]]

[[Category: Beta-sandwich]]

[[Category: Exoglycosidase]]

[[Category: Glycoside hydrolase]]

[[Category: Hydrolase]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5iaz is ON HOLD  until Paper Publication
+==The C-terminal domain of rice beta-galactosidase 1==
+<StructureSection load='5iaz' size='340' side='right' caption='[[5iaz]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5iaz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IAZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IAZ FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iaz OCA], [http://pdbe.org/5iaz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iaz RCSB], [http://www.ebi.ac.uk/pdbsum/5iaz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iaz ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Most plant beta-galactosidases, which belong to glycoside hydrolase family 35, have a C-terminal domain homologous to animal galactose and rhamnose-binding lectins. To investigate the structure and function of this domain, the C-terminal domain of the rice (Oryza sativa L.) beta-galactosidase 1 (OsBGal1 Cter) was expressed in Escherichia coli and purified to homogeneity. The free OsBGal1 Cter is monomeric with a native molecular weight of 15kDa. NMR spectroscopy indicated that OsBGal1 Cter comprises five beta-strands and one alpha-helix. The structure of this domain is similar to lectin domains from animals, but loops A and C of OsBGal1 Cter are longer than the corresponding loops from related animal lectins with known structures. In addition, loop A of OsBGal1 Cter was not well defined, suggesting it is flexible. Although OsBGal1 Cter was predicted to be a galactose/rhamnose-binding domain, binding with rhamnose, galactose, glucose, beta-1,4-d-galactobiose and raffinose could not be observed in NMR experiments.
-Authors: Rimlumduan, T., Hua, Y.-l., Tanaka, T., Ketudat-Cairns, J.R.
+Structure of a plant beta-galactosidase C-terminal domain.,Rimlumduan T, Hua YL, Tanaka T, Ketudat Cairns JR Biochim Biophys Acta. 2016 Jul 22;1864(10):1411-1418. doi:, 10.1016/j.bbapap.2016.07.005. PMID:27451952<ref>PMID:27451952</ref>
-Description: The C-terminal domain of rice beta-galactosidase 1
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Hua, Y.-L]]
+<div class="pdbe-citations 5iaz" style="background-color:#fffaf0;"></div>
-[[Category: Ketudat-Cairns, J.R]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Hua, Y l]]
+[[Category: Ketudat-Cairns, J R]]
 [[Category: Rimlumduan, T]]
 [[Category: Tanaka, T]]
+[[Category: Beta-sandwich]]
+[[Category: Exoglycosidase]]
+[[Category: Glycoside hydrolase]]
+[[Category: Hydrolase]]