1o97: Difference between revisions
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|PDB= 1o97 |SIZE=350|CAPTION= <scene name='initialview01'>1o97</scene>, resolution 1.60Å | |PDB= 1o97 |SIZE=350|CAPTION= <scene name='initialview01'>1o97</scene>, resolution 1.60Å | ||
|SITE= <scene name='pdbsite=AMP:Fad+Binding+Site+For+Chain+D'>AMP</scene> | |SITE= <scene name='pdbsite=AMP:Fad+Binding+Site+For+Chain+D'>AMP</scene> | ||
|LIGAND= <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene> | |LIGAND= <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o97 OCA], [http://www.ebi.ac.uk/pdbsum/1o97 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1o97 RCSB]</span> | |||
}} | }} | ||
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[[Category: Sutcliffe, M J.]] | [[Category: Sutcliffe, M J.]] | ||
[[Category: Talfournier, F.]] | [[Category: Talfournier, F.]] | ||
[[Category: electron transfer]] | [[Category: electron transfer]] | ||
[[Category: fad binding]] | [[Category: fad binding]] | ||
[[Category: flavoprotein]] | [[Category: flavoprotein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:41:44 2008'' | ||
Revision as of 19:41, 30 March 2008
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| 1o97, resolution 1.60Å | |||||||||||||
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| Sites: | AMP | ||||||||||||
| Ligands: | AMP, FAD | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
STRUCTURE OF ELECTRON TRANSFERRING FLAVOPROTEIN FROM METHYLOPHILUS METHYLOTROPHUS, RECOGNITION LOOP REMOVED BY LIMITED PROTEOLYSIS
Overview
Here we report the crystal structures of a ternary electron transfer complex showing extensive motion at the protein interface. This physiological complex comprises the iron-sulfur flavoprotein trimethylamine dehydrogenase and electron transferring flavoprotein (ETF) from Methylophilus methylotrophus. In addition, we report the crystal structure of free ETF. In the complex, electron density for the FAD domain of ETF is absent, indicating high mobility. Positions for the FAD domain are revealed by molecular dynamics simulation, consistent with crystal structures and kinetic data. A dual interaction of ETF with trimethylamine dehydrogenase provides for dynamical motion at the protein interface: one site acts as an anchor, thereby allowing the other site to sample a large range of interactions, some compatible with rapid electron transfer. This study establishes the role of conformational sampling in multi-domain redox systems, providing insight into electron transfer between ETFs and structurally distinct redox partners.
About this Structure
1O97 is a Protein complex structure of sequences from Methylophilus methylotrophus. Full crystallographic information is available from OCA.
Reference
Extensive conformational sampling in a ternary electron transfer complex., Leys D, Basran J, Talfournier F, Sutcliffe MJ, Scrutton NS, Nat Struct Biol. 2003 Mar;10(3):219-25. PMID:12567183
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