|
|
| Line 1: |
Line 1: |
| | '''Unreleased structure''' |
|
| |
|
| ==Crystal structure of KAI2-like protein from Striga (apo state 1)==
| | The entry 5dnw is ON HOLD until Paper Publication |
| <StructureSection load='5dnw' size='340' side='right' caption='[[5dnw]], [[Resolution|resolution]] 2.02Å' scene=''>
| |
| == Structural highlights ==
| |
| <table><tr><td colspan='2'>[[5dnw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DNW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DNW FirstGlance]. <br>
| |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
| |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5dnu|5dnu]], [[5dnv|5dnv]]</td></tr>
| |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dnw OCA], [http://pdbe.org/5dnw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dnw RCSB], [http://www.ebi.ac.uk/pdbsum/5dnw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dnw ProSAT]</span></td></tr>
| |
| </table>
| |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
| |
| The perception of two plant germination inducers, karrikins and strigolactones, are mediated by the proteins KAI2 and D14. Recently, KAI2-type proteins from parasitic weeds, which are possibly related to seed germination induced by strigolactone, have been classified into three clades characterized by different responses to karrikin/strigolactone. Here we characterized a karrikin-binding protein in Striga (ShKAI2iB) that belongs to intermediate-evolving KAI2 and provided the structural bases for its karrikin-binding specificity. Binding assays showed that ShKAI2iB bound karrikins but not strigolactone, differing from other KAI2 and D14. The crystal structures of ShKAI2iB and ShKAI2iB-karrikin complex revealed obvious structural differences in a helix located at the entry of its ligand-binding cavity. This results in a smaller closed pocket, which is also the major cause of ShKAI2iB's specificity of binding karrikin. Our structural study also revealed that a few non-conserved amino acids led to the distinct ligand-binding profile of ShKAI2iB, suggesting that the evolution of KAI2 resulted in its diverse functions.
| |
|
| |
|
| Structural basis of unique ligand specificity of KAI2-like protein from parasitic weed Striga hermonthica.,Xu Y, Miyakawa T, Nakamura H, Nakamura A, Imamura Y, Asami T, Tanokura M Sci Rep. 2016 Aug 10;6:31386. doi: 10.1038/srep31386. PMID:27507097<ref>PMID:27507097</ref>
| | Authors: |
|
| |
|
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| | Description: |
| </div>
| | [[Category: Unreleased Structures]] |
| <div class="pdbe-citations 5dnw" style="background-color:#fffaf0;"></div>
| |
| == References ==
| |
| <references/>
| |
| __TOC__
| |
| </StructureSection>
| |
| [[Category: Miyakawa, T]]
| |
| [[Category: Nakamura, A]]
| |
| [[Category: Tanokura, M]]
| |
| [[Category: Xu, Y]]
| |
| [[Category: Hydrolase]]
| |
| [[Category: Kai2 karrikin striga]] | |
Unreleased structure
The entry 5dnw is ON HOLD until Paper Publication
Authors:
Description:
