5g4u: Difference between revisions
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==Association of three two-k-turn units based on Kt-7 3bU,3nU, forming a triangular-shaped structure== | |||
<StructureSection load='5g4u' size='340' side='right' caption='[[5g4u]], [[Resolution|resolution]] 2.65Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5g4u]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G4U FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5g4t|5g4t]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g4u OCA], [http://pdbe.org/5g4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g4u RCSB], [http://www.ebi.ac.uk/pdbsum/5g4u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g4u ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/RL7A_ARCFU RL7A_ARCFU]] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA and box C/D sRNAs.<ref>PMID:12560482</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
k-Turns are widespread RNA architectural elements that mediate tertiary interactions. We describe a double-kink-turn motif comprising two inverted k-turns that forms a tight horse-shoe structure that can assemble into a variety of shapes by coaxial association of helical ends. Using X-ray crystallography we show that these assemble with two (dumbell), three (triangle) and four units (square), with or without bound protein, within the crystal lattice. In addition, exchange of a single basepair can almost double the pore radius or shape of a molecular assembly. On the basis of this analysis we synthesized a 114 nt self-complementary RNA containing six k-turns. The crystal structure of this species shows that it forms a quasi-cyclic triangular object. These are randomly disposed about the three-fold axis in the crystal lattice, generating a circular RNA of quasi D3 symmetry with a shape reminiscent of that of a cyclohexane molecule in its chair conformation. This work demonstrates that the k-turn is a powerful building block in the construction of nano-scale molecular objects, and illustrates why k-turns are widely used in natural RNA molecules to organize long-range architecture and mediate tertiary contacts. | |||
A quasi-cyclic RNA nano-scale molecular object constructed using kink turns.,Huang L, Lilley DM Nanoscale. 2016 Aug 18;8(33):15189-95. doi: 10.1039/c6nr05186c. PMID:27506301<ref>PMID:27506301</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5g4u" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Huang, L]] | [[Category: Huang, L]] | ||
[[Category: Lilley, D M.J]] | |||
[[Category: Kink turn]] | |||
[[Category: L7ae]] | |||
[[Category: Nano crystal packing]] | |||
[[Category: Nanotechnology]] | |||
[[Category: Rna]] | |||
[[Category: Rna-protein complex]] | |||
