Samatey/3: Difference between revisions

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In actuality, the hook is assembled by the flagellar basal body, not in a vacuum. Hook monomers are believed to be secreted through a central channel in the basal body prior to assembly, as shown in a movie available at [[Flagella, bacterial|Bacterial Flagella]].

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==Caulobacter Hook Model==

A model of the hook of ''Caulobacter crecentus'' was made by manually docking the crystallographic structure of FlgE-Cc32 domains D2 and D3 into the 20 Å electron density map published in 1981<ref>PMID: 7338902</ref>. Here you can <scene name='47/478822/Caucr-hook-cao/1'>view this hook model</scene> (alpha carbons only, spacefilled to 3.5 Å<ref>The ''Caulobacter crecentus'' hook model contains 55 copies of the monomer, for a total of 121,055 non-hydrogen atoms. There are 16,665 alpha carbon atoms in the model. The van der Waals radius of carbon is 1.7 Å. In order to make the FlgE domains look solid, the alpha carbons are displayed with a radius of 3.5 Å.</ref>).

Most of the large cavity is filled by domains D0 and D1, but these domains are missing in this model.

Each of the 11 protofilaments has a distinct color, so there are 11 colors. Here, <scene name='47/478822/Caucr-hook-cao/2'>one protofilament is marked with a black line</scene>. Each protofilament forms one of the 11-start helices.

There is a large space between domains D2+D3 '''within''' a protofilament. Thus, protofilaments are held together by interactions between D0 and D1 (missing in this model). However, domains D2+D3 interact '''between''' protofilaments, forming 6 helices. Here, <scene name='47/478822/Caucr-hook-cao/3'>one of the 6-start helices is marked with a red line, and the 6 starts are numbered</scene>.

<hr>

</StructureSection>

Samatey/3: Difference between revisions

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@@ Line 93: / Line 93: @@
 In actuality, the hook is assembled by the flagellar basal body, not in a vacuum. Hook monomers are believed to be secreted through a central channel in the basal body prior to assembly, as shown in a movie available at [[Flagella, bacterial|Bacterial Flagella]].
 -->
+==Caulobacter Hook Model==
+A model of the hook of ''Caulobacter crecentus'' was made by manually docking the crystallographic structure of FlgE-Cc32 domains D2 and D3 into the 20 &Aring; electron density map published in 1981<ref>PMID: 7338902</ref>. Here you can <scene name='47/478822/Caucr-hook-cao/1'>view this hook model</scene> (alpha carbons only, spacefilled to 3.5 &Aring;<ref>The ''Caulobacter crecentus'' hook model contains 55 copies of the monomer, for a total of 121,055 non-hydrogen atoms. There are 16,665 alpha carbon atoms in the model. The van der Waals radius of carbon is 1.7 &Aring;. In order to make the FlgE domains look solid, the alpha carbons are displayed with a radius of 3.5 &Aring;.</ref>).
+Most of the large cavity is filled by domains D0 and D1, but these domains are missing in this model.
+Each of the 11 protofilaments has a distinct color, so there are 11 colors. Here, <scene name='47/478822/Caucr-hook-cao/2'>one protofilament is marked with a black line</scene>. Each protofilament forms one of the 11-start helices.
+There is a large space between domains D2+D3 '''within''' a protofilament. Thus, protofilaments are held together by interactions between D0 and D1 (missing in this model). However, domains D2+D3 interact '''between''' protofilaments, forming 6 helices. Here, <scene name='47/478822/Caucr-hook-cao/3'>one of the 6-start helices is marked with a red line, and the 6 starts are numbered</scene>.
 <hr>
 </StructureSection>