5t3l: Difference between revisions
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==B-DNA (CGCGAATTCGCG)2 soaked with selenourea for 1 min== | |||
<StructureSection load='5t3l' size='340' side='right' caption='[[5t3l]], [[Resolution|resolution]] 1.58Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5t3l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T3L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T3L FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEY:SELENOUREA'>SEY</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t3l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t3l OCA], [http://pdbe.org/5t3l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t3l RCSB], [http://www.ebi.ac.uk/pdbsum/5t3l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t3l ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Majority of novel X-ray crystal structures of proteins are currently solved using the anomalous diffraction signal provided by selenium after incorporation of selenomethionine instead of natural methionine by genetic engineering methods. However, selenium can be inserted into protein crystals in the form of selenourea (SeC(NH2)2), by adding the crystalline powder of selenourea into mother liquor or cryo-solution with native crystals, in analogy to the classic procedure of heavy-atom derivatization. Selenourea is able to bind to reactive groups at the surface of macromolecules primarily through hydrogen bonds, where the selenium atom may serve as acceptor and amide groups as donors. Selenourea has different chemical properties than heavy-atom reagents and halide ions and provides a convenient way of phasing crystal structures of macromolecules. | |||
Selenourea: a convenient phasing vehicle for macromolecular X-ray crystal structures.,Luo Z Sci Rep. 2016 Nov 14;6:37123. doi: 10.1038/srep37123. PMID:27841370<ref>PMID:27841370</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5t3l" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Dauter, Z]] | [[Category: Dauter, Z]] | ||
[[Category: Luo, Z]] | [[Category: Luo, Z]] | ||
[[Category: B-dna]] | |||
[[Category: Dna]] | |||
[[Category: Selenourea]] | |||
Revision as of 18:38, 10 December 2016
B-DNA (CGCGAATTCGCG)2 soaked with selenourea for 1 min
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