5ks2: Difference between revisions

Revision as of 16:46, 4 January 2017

RAWV_CTD (Helix form) of 16S/23S 2'-O-methyltransferase TlyA

Structural highlights

5ks2 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	CL
Related:	5eov
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TLYA_MYCTU] Acts as a host evasion factor, that significantly contributes to the pathogenesis of M.tuberculosis by modulating adaptive immune responses by inhibiting host-protective Th1 and Th17 cytokine responses as well as autophagy (PubMed:25847237). Catalyzes the 2'-O-methylation at nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA (PubMed:16857584, PubMed:20854656). Is likely involved in ribosomal biogenesis (PubMed:21443791). Also exhibits hemolytic activity in vitro, by binding with and oligomerizing into host cell membranes (PubMed:20854656, PubMed:9611795).^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Johansen SK, Maus CE, Plikaytis BB, Douthwaite S. Capreomycin binds across the ribosomal subunit interface using tlyA-encoded 2'-O-methylations in 16S and 23S rRNAs. Mol Cell. 2006 Jul 21;23(2):173-82. PMID:16857584 doi:https://dx.doi.org/10.1016/j.molcel.2006.05.044
↑ Rahman A, Srivastava SS, Sneh A, Ahmed N, Krishnasastry MV. Molecular characterization of tlyA gene product, Rv1694 of Mycobacterium tuberculosis: a non-conventional hemolysin and a ribosomal RNA methyl transferase. BMC Biochem. 2010 Sep 20;11:35. doi: 10.1186/1471-2091-11-35. PMID:20854656 doi:https://dx.doi.org/10.1186/1471-2091-11-35
↑ Arenas NE, Salazar LM, Soto CY, Vizcaino C, Patarroyo ME, Patarroyo MA, Gomez A. Molecular modeling and in silico characterization of Mycobacterium tuberculosis TlyA: possible misannotation of this tubercle bacilli-hemolysin. BMC Struct Biol. 2011 Mar 28;11:16. doi: 10.1186/1472-6807-11-16. PMID:21443791 doi:https://dx.doi.org/10.1186/1472-6807-11-16
↑ Rahman MA, Sobia P, Dwivedi VP, Bhawsar A, Singh DK, Sharma P, Moodley P, Van Kaer L, Bishai WR, Das G. Mycobacterium tuberculosis TlyA Protein Negatively Regulates T Helper (Th) 1 and Th17 Differentiation and Promotes Tuberculosis Pathogenesis. J Biol Chem. 2015 Jun 5;290(23):14407-17. doi: 10.1074/jbc.M115.653600. Epub 2015, Apr 6. PMID:25847237 doi:https://dx.doi.org/10.1074/jbc.M115.653600
↑ Wren BW, Stabler RA, Das SS, Butcher PD, Mangan JA, Clarke JD, Casali N, Parish T, Stoker NG. Characterization of a haemolysin from Mycobacterium tuberculosis with homology to a virulence factor of Serpulina hyodysenteriae. Microbiology. 1998 May;144 ( Pt 5):1205-11. PMID:9611795

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OCA

[1] Johansen SK, Maus CE, Plikaytis BB, Douthwaite S. Capreomycin binds across the ribosomal subunit interface using tlyA-encoded 2'-O-methylations in 16S and 23S rRNAs. Mol Cell. 2006 Jul 21;23(2):173-82. PMID:16857584 doi:https://dx.doi.org/10.1016/j.molcel.2006.05.044

[2] Rahman A, Srivastava SS, Sneh A, Ahmed N, Krishnasastry MV. Molecular characterization of tlyA gene product, Rv1694 of Mycobacterium tuberculosis: a non-conventional hemolysin and a ribosomal RNA methyl transferase. BMC Biochem. 2010 Sep 20;11:35. doi: 10.1186/1471-2091-11-35. PMID:20854656 doi:https://dx.doi.org/10.1186/1471-2091-11-35

[3] Arenas NE, Salazar LM, Soto CY, Vizcaino C, Patarroyo ME, Patarroyo MA, Gomez A. Molecular modeling and in silico characterization of Mycobacterium tuberculosis TlyA: possible misannotation of this tubercle bacilli-hemolysin. BMC Struct Biol. 2011 Mar 28;11:16. doi: 10.1186/1472-6807-11-16. PMID:21443791 doi:https://dx.doi.org/10.1186/1472-6807-11-16

[4] Rahman MA, Sobia P, Dwivedi VP, Bhawsar A, Singh DK, Sharma P, Moodley P, Van Kaer L, Bishai WR, Das G. Mycobacterium tuberculosis TlyA Protein Negatively Regulates T Helper (Th) 1 and Th17 Differentiation and Promotes Tuberculosis Pathogenesis. J Biol Chem. 2015 Jun 5;290(23):14407-17. doi: 10.1074/jbc.M115.653600. Epub 2015, Apr 6. PMID:25847237 doi:https://dx.doi.org/10.1074/jbc.M115.653600

[5] Wren BW, Stabler RA, Das SS, Butcher PD, Mangan JA, Clarke JD, Casali N, Parish T, Stoker NG. Characterization of a haemolysin from Mycobacterium tuberculosis with homology to a virulence factor of Serpulina hyodysenteriae. Microbiology. 1998 May;144 ( Pt 5):1205-11. PMID:9611795

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5ks2 is ON HOLD  until Paper Publication
+==RAWV_CTD (Helix form) of 16S/23S 2'-O-methyltransferase TlyA==
+<StructureSection load='5ks2' size='340' side='right' caption='[[5ks2]], [[Resolution|resolution]] 2.18&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5ks2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KS2 FirstGlance]. <br>
-Description:
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-[[Category: Unreleased Structures]]
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5eov|5eov]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ks2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ks2 OCA], [http://pdbe.org/5ks2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ks2 RCSB], [http://www.ebi.ac.uk/pdbsum/5ks2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ks2 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/TLYA_MYCTU TLYA_MYCTU]] Acts as a host evasion factor, that significantly contributes to the pathogenesis of M.tuberculosis by modulating adaptive immune responses by inhibiting host-protective Th1 and Th17 cytokine responses as well as autophagy (PubMed:25847237). Catalyzes the 2'-O-methylation at nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA (PubMed:16857584, PubMed:20854656). Is likely involved in ribosomal biogenesis (PubMed:21443791). Also exhibits hemolytic activity in vitro, by binding with and oligomerizing into host cell membranes (PubMed:20854656, PubMed:9611795).<ref>PMID:16857584</ref> <ref>PMID:20854656</ref> <ref>PMID:21443791</ref> <ref>PMID:25847237</ref> <ref>PMID:9611795</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Conn, G L]]
+[[Category: Kuiper, E G]]
+[[Category: Antibiotic sensitivity]]
+[[Category: Hemolysin]]
+[[Category: Methyltransferase]]
+[[Category: Ribosome]]
+[[Category: Transferase]]

5ks2: Difference between revisions

Revision as of 16:46, 4 January 2017

RAWV_CTD (Helix form) of 16S/23S 2'-O-methyltransferase TlyA

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