OBrochtasSandbox: Difference between revisions
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Most but not all of the actual and predicted mutations are <scene name='75/750300/Tspred_rosett_burried_residues/1'>buried</scene>. Note that T1221 and G1000S are not buried. | Most but not all of the actual and predicted mutations are <scene name='75/750300/Tspred_rosett_burried_residues/1'>buried</scene>. Note that T1221 and G1000S are not buried. | ||
Here are the <scene name='75/750300/Tspred_rosett_hydroph_residues/1'>hydrophobic residues</scene>. | |||
Revision as of 19:41, 16 January 2017
Anopheles arabiensis Insulin Receptor like Protein
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The Anopheles arabiensis Insulin Receptor's kinase domain was modeled by Brian Pierce for the 'An arab ts lethal project'
This shows the basic structure of the kinase domain with an N and C lobe. The ATP will sit between them just below Beta 1
This shows the ATP binding site (purple) and the catalytic loop (green). Here it is as a spacefilled model.
These are three ts mutations found in other RTKs.
G1000S (magenta), A1133G (orange), T1221I (teal)
And as a spacefilled model
This threonine when mutated to isoleucine resulted in ts lethal mutations in the sevenless and EGFR-like genes in D. melanogster
Using the software TS_pred (a ts mutation prediction program): these are the 5 best sites to mutate based on sequence and structure - L1236, I1071, V1196, L1197,I1258 (red) Using Rosetta_ts_rbf (another ts mutation prediction program): these are the top 5 sites -W1173P, M1075K, Y1208G, L1197P, Y1208Asn (yellow)
Both TS_pred and Rosetta_ts_rbf had L1197 in their top 5 (black)
Most but not all of the actual and predicted mutations are buried. Note that T1221 and G1000S are not buried.
Here are the hydrophobic residues.
