1s77: Difference between revisions
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|PDB= 1s77 |SIZE=350|CAPTION= <scene name='initialview01'>1s77</scene>, resolution 2.69Å | |PDB= 1s77 |SIZE=350|CAPTION= <scene name='initialview01'>1s77</scene>, resolution 2.69Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> | |LIGAND= <scene name='pdbligand=3DA:3'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>3DA</scene>, <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span> | ||
|GENE= 1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | |GENE= 1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 Enterobacteria phage T7]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s77 OCA], [http://www.ebi.ac.uk/pdbsum/1s77 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s77 RCSB]</span> | |||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
1S77 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | 1S77 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S77 OCA]. | ||
==Reference== | ==Reference== | ||
The structural mechanism of translocation and helicase activity in T7 RNA polymerase., Yin YW, Steitz TA, Cell. 2004 Feb 6;116(3):393-404. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15016374 15016374] | The structural mechanism of translocation and helicase activity in T7 RNA polymerase., Yin YW, Steitz TA, Cell. 2004 Feb 6;116(3):393-404. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15016374 15016374] | ||
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Enterobacteria phage t7]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Steitz, T A.]] | [[Category: Steitz, T A.]] | ||
[[Category: Yin, Y W.]] | [[Category: Yin, Y W.]] | ||
[[Category: t7 rna polymerase]] | [[Category: t7 rna polymerase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:38:21 2008'' | ||
Revision as of 20:38, 30 March 2008
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| 1s77, resolution 2.69Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | 3DA, A, C, DA, DC, DG, DT, G, MG, POP | ||||||||||||
| Gene: | 1 (Enterobacteria phage T7) | ||||||||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
T7 RNAP product pyrophosphate elongation complex
Overview
RNA polymerase functions like a molecular motor that can convert chemical energy into the work of strand separation and translocation along the DNA during transcription. The structures of phage T7 RNA polymerase in an elongation phase substrate complex that includes the incoming nucleoside triphosphate and a pretranslocation product complex that includes the product pyrophosphate (PPi) are described here. These structures and the previously determined posttranslocation elongation complex demonstrate that two enzyme conformations exist during a cycle of single nucleotide addition. One orientation of a five-helix subdomain is stabilized by the phosphates of either the incoming NTP or by the product PPi. A second orientation of this subdomain is stable in their absence and is associated with translocation of the heteroduplex product as well as strand separation of the downstream DNA. We propose that the dissociation of the product PPi after nucleotide addition produces the protein conformational change resulting in translocation and strand separation.
About this Structure
1S77 is a Single protein structure of sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA.
Reference
The structural mechanism of translocation and helicase activity in T7 RNA polymerase., Yin YW, Steitz TA, Cell. 2004 Feb 6;116(3):393-404. PMID:15016374
Page seeded by OCA on Sun Mar 30 23:38:21 2008
