5d99: Difference between revisions

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==3DW4 redetermined ~~ab initio~~ by direct methods==

==3DW4 redetermined by direct methods starting from random phase angles==

== Structural highlights ==

Line 7:

<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3dw4|3dw4]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d99 OCA], [http://pdbe.org/5d99 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d99 RCSB], [http://www.ebi.ac.uk/pdbsum/5d99 PDBsum]</span></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d99 OCA], [http://pdbe.org/5d99 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d99 RCSB], [http://www.ebi.ac.uk/pdbsum/5d99 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d99 ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Site-specific 2'-methylseleno RNA labeling is a promising tool for tackling the phase problem in RNA crystallography. We have developed an efficient strategy for crystallization and structure determination of RNA and RNA/protein complexes based on preliminary crystallization screening of 2'-OCH(3)-modified RNA sequences, prior to the replacement of 2'-OCH(3) groups with their 2'-SeCH(3) counterparts. The method exploits the similar crystallization properties of 2'-OCH(3)- and 2'-SeCH(3)-modified RNAs and has been successfully validated for two test cases. In addition, our data show that 2'-SeCH(3)-modified RNA have an increased resistance to X-ray radiolysis in comparison with commonly used 5-halogen-modified RNA, which permits collection of experimental electron density maps of remarkable quality.

A fast selenium derivatization strategy for crystallization and phasing of RNA structures.,Olieric V, Rieder U, Lang K, Serganov A, Schulze-Briese C, Micura R, Dumas P, Ennifar E RNA. 2009 Apr;15(4):707-15. Epub 2009 Feb 18. PMID:19228585<ref>PMID:19228585</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

__TOC__

</StructureSection>

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@@ Line 1: / Line 1: @@
-==3DW4 redetermined ab initio by direct methods==
+==3DW4 redetermined by direct methods starting from random phase angles==
 <StructureSection load='5d99' size='340' side='right' caption='[[5d99]], [[Resolution|resolution]] 0.97&Aring;' scene=''>
 == Structural highlights ==
@@ Line 7: / Line 7: @@
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3dw4|3dw4]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d99 OCA], [http://pdbe.org/5d99 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d99 RCSB], [http://www.ebi.ac.uk/pdbsum/5d99 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d99 OCA], [http://pdbe.org/5d99 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d99 RCSB], [http://www.ebi.ac.uk/pdbsum/5d99 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d99 ProSAT]</span></td></tr>
 </table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Site-specific 2'-methylseleno RNA labeling is a promising tool for tackling the phase problem in RNA crystallography. We have developed an efficient strategy for crystallization and structure determination of RNA and RNA/protein complexes based on preliminary crystallization screening of 2'-OCH(3)-modified RNA sequences, prior to the replacement of 2'-OCH(3) groups with their 2'-SeCH(3) counterparts. The method exploits the similar crystallization properties of 2'-OCH(3)- and 2'-SeCH(3)-modified RNAs and has been successfully validated for two test cases. In addition, our data show that 2'-SeCH(3)-modified RNA have an increased resistance to X-ray radiolysis in comparison with commonly used 5-halogen-modified RNA, which permits collection of experimental electron density maps of remarkable quality.
+A fast selenium derivatization strategy for crystallization and phasing of RNA structures.,Olieric V, Rieder U, Lang K, Serganov A, Schulze-Briese C, Micura R, Dumas P, Ennifar E RNA. 2009 Apr;15(4):707-15. Epub 2009 Feb 18. PMID:19228585<ref>PMID:19228585</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 5d99" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
 __TOC__
 </StructureSection>