4z2f: Difference between revisions

Revision as of 13:46, 5 April 2017

Sclerotium Rolfsii lectin variant (SSR2) with fine carbohydrate specificity

Structural highlights

4z2f is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	MPD
Related:	4yld
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

SRL is a cell wall associated developmental-stage specific lectin secreted by Sclerotium rolfsii, a soil-born pathogenic fungus. SRL displays specificity for TF antigen (Galbeta1-->3GalNAc-alpha-Ser//Thr) expressed in all cancer types and has tumour suppressing effects in vivo. Considering the immense potential of SRL in cancer research, we have generated two variant gene constructs of SRL and expressed in E. coli to refine the sugar specificity and solubility by altering the surface charge. SSR1 and SSR2 are two different recombinant variants of SRL, both of which recognize TF antigen but only SSR1 binds to Tn antigen (GalNAcalpha-Ser/Thr). The glycan array analysis of the variants demonstrated that SSR1 recognizes TF antigen and their derivative with high affinity similar to SRL but showed highest affinity towards the sialylated Tn antigen, unlike SRL. The carbohydrate binding property of SSR2 remains unaltered compared to SRL. The crystal structures of the two variants were determined in free form and in complex with N-acetylglucosamine at 1.7 A and 1.6 A resolution, respectively. Structural analysis highlighted the structural basis of the fine carbohydrate specificity of the two SRL variants and results are in agreement with glycan array analysis.

Molecular Cloning, Carbohydrate Specificity and the Crystal Structure of Two Sclerotium rolfsii Lectin Variants.,Peppa VI, Venkat H, Kantsadi AL, Inamdar SR, Bhat GG, Eligar S, Shivanand A, Chachadi VB, Satisha GJ, Swamy BM, Skamnaki VT, Zographos SE, Leonidas DD Molecules. 2015 Jun 12;20(6):10848-65. doi: 10.3390/molecules200610848. PMID:26076107^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Peppa VI, Venkat H, Kantsadi AL, Inamdar SR, Bhat GG, Eligar S, Shivanand A, Chachadi VB, Satisha GJ, Swamy BM, Skamnaki VT, Zographos SE, Leonidas DD. Molecular Cloning, Carbohydrate Specificity and the Crystal Structure of Two Sclerotium rolfsii Lectin Variants. Molecules. 2015 Jun 12;20(6):10848-65. doi: 10.3390/molecules200610848. PMID:26076107 doi:https://dx.doi.org/10.3390/molecules200610848

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OCA

[1] Peppa VI, Venkat H, Kantsadi AL, Inamdar SR, Bhat GG, Eligar S, Shivanand A, Chachadi VB, Satisha GJ, Swamy BM, Skamnaki VT, Zographos SE, Leonidas DD. Molecular Cloning, Carbohydrate Specificity and the Crystal Structure of Two Sclerotium rolfsii Lectin Variants. Molecules. 2015 Jun 12;20(6):10848-65. doi: 10.3390/molecules200610848. PMID:26076107 doi:https://dx.doi.org/10.3390/molecules200610848

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 ==Sclerotium Rolfsii lectin variant (SSR2) with fine carbohydrate specificity==
 <StructureSection load='4z2f' size='340' side='right' caption='[[4z2f]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4yld|4yld]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z2f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4z2f RCSB], [http://www.ebi.ac.uk/pdbsum/4z2f PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z2f OCA], [http://pdbe.org/4z2f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z2f RCSB], [http://www.ebi.ac.uk/pdbsum/4z2f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z2f ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 15: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4z2f" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4z2f: Difference between revisions

Revision as of 13:46, 5 April 2017

Sclerotium Rolfsii lectin variant (SSR2) with fine carbohydrate specificity

Structural highlights

Publication Abstract from PubMed

References

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