5gt9: Difference between revisions
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The | ==The X-ray structure of 7beta-hydroxysteroid dehydrogenase== | ||
<StructureSection load='5gt9' size='340' side='right' caption='[[5gt9]], [[Resolution|resolution]] 1.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5gt9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GT9 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gt9 OCA], [http://pdbe.org/5gt9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gt9 RCSB], [http://www.ebi.ac.uk/pdbsum/5gt9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gt9 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In mammals, bile acids/salts and their glycine and taurine conjugates are effectively recycled through enterohepatic circulation. 7beta-Hydroxysteroid dehydrogenases (7beta-HSDHs; EC 1.1.1.201), including that from the intestinal microbe Collinsella aerofaciens, catalyse the NADPH-dependent reversible oxidation of secondary bile-acid products to avoid potential toxicity. Here, the first structure of NADP+ bound to dimeric 7beta-HSDH is presented. In one active site, NADP+ adopts a conventional binding mode similar to that displayed in related enzyme structures. However, in the other active site a unique binding mode is observed in which the orientation of the nicotinamide is different. Since 7beta-HSDH has become an attractive target owing to the wide and important pharmaceutical use of its product ursodeoxycholic acid, this work provides a more detailed template to support rational protein engineering to improve the enzymatic activities of this useful biocatalyst, further improving the yield of ursodeoxycholic acid and its other applications. | |||
Structure of NADP+-bound 7beta-hydroxysteroid dehydrogenase reveals two cofactor-binding modes.,Wang R, Wu J, Jin DK, Chen Y, Lv Z, Chen Q, Miao Q, Huo X, Wang F Acta Crystallogr F Struct Biol Commun. 2017 May 1;73(Pt 5):246-252. doi:, 10.1107/S2053230X17004460. Epub 2017 Apr 26. PMID:28471355<ref>PMID:28471355</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5gt9" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Chen, Q]] | [[Category: Chen, Q]] | ||
[[Category: Huo, X]] | |||
[[Category: Lv, Z]] | [[Category: Lv, Z]] | ||
[[Category: | [[Category: Wang, F]] | ||
[[Category: Wang, R]] | |||
[[Category: Oxidoreductase]] | |||
[[Category: Short chain dehydrogenase]] | |||
[[Category: Steroid substrate]] | |||
Revision as of 15:44, 17 May 2017
The X-ray structure of 7beta-hydroxysteroid dehydrogenase
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