5m60: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
Line 1: Line 1:
'''Unreleased structure'''


The entry 5m60 is ON HOLD  until Paper Publication
==Chaetomium thermophilum beta-1-3-glucanase==
<StructureSection load='5m60' size='340' side='right' caption='[[5m60]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5m60]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M60 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M60 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m60 OCA], [http://pdbe.org/5m60 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m60 RCSB], [http://www.ebi.ac.uk/pdbsum/5m60 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m60 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Crystal structures of a beta-1,3-glucanase from the thermophilic fungus Chaetomium thermophilum were determined at 1.20 and 1.42A resolution in the free and glucose-bound form, respectively. This is the third structure of a family 55 glycoside hydrolase (GH55) member and the second from a fungus. Based on comparative structural studies and site-directed mutagenesis, Glu654 is proposed as the catalytic acid residue. The substrate binding cleft exhibits restricted access on one side, rendering the enzyme as an exo-beta-1,3-glucanase as confirmed also by thin layer chromatography experiments. A lack of stacking interactions was found at the substrate binding cleft, suggesting that interactions at positions -1, +1 and +2 are sufficient to orientate the substrate. A binding pocket was identified that could explain binding of branched laminarin and accumulation of laminaritriose.


Authors: Papageorgiou, A.C., Chen, J., Li, D.
Crystal structure and biological implications of a glycoside hydrolase family 55 beta-1,3-glucanase from Chaetomium thermophilum.,Papageorgiou AC, Chen J, Li D Biochim Biophys Acta. 2017 May 4. pii: S1570-9639(17)30084-5. doi:, 10.1016/j.bbapap.2017.05.002. PMID:28479293<ref>PMID:28479293</ref>


Description: Chaetomium thermophilum beta-1-3-glucanase
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 5m60" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Chen, J]]
[[Category: Li, D]]
[[Category: Li, D]]
[[Category: Papageorgiou, A.C]]
[[Category: Papageorgiou, A C]]
[[Category: Chen, J]]
[[Category: Cellulose]]
[[Category: Fungus]]
[[Category: Glucanase]]
[[Category: Glucan]]
[[Category: Thermostability]]
[[Category: Transferase]]
Retrieved from "https://proteopedia.org/5m60"