5vg0: Difference between revisions

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'''Unreleased structure'''


The entry 5vg0 is ON HOLD  until Paper Publication
==Room temperature X-ray crystallographic structure of a Jonesia denitrificans lytic polysaccharide monooxygenase at 1.1 angstrom resolution.==
<StructureSection load='5vg0' size='340' side='right' caption='[[5vg0]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5vg0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VG0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VG0 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=PER:PEROXIDE+ION'>PER</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5vg1|5vg1]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vg0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vg0 OCA], [http://pdbe.org/5vg0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vg0 RCSB], [http://www.ebi.ac.uk/pdbsum/5vg0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vg0 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A 1.1 A resolution, room-temperature X-ray structure and a 2.1 A resolution neutron structure of a chitin-degrading lytic polysaccharide monooxygenase domain from the bacterium Jonesia denitrificans (JdLPMO10A) show a putative dioxygen species equatorially bound to the active site copper. Both structures show an elongated density for the dioxygen, most consistent with a Cu(II)-bound peroxide. The coordination environment is consistent with Cu(II). In the neutron and X-ray structures, difference maps reveal the N-terminal amino group, involved in copper coordination, is present as a mixed ND2 and ND-, suggesting a role for the copper ion in shifting the pKa of the amino terminus.


Authors: Bacik, J.-P., Unkefer, C.J., Chen, J.C.H.
Neutron and Atomic Resolution X-ray Structures of a Lytic Polysaccharide Monooxygenase Reveal Copper-Mediated Dioxygen Binding and Evidence for N-Terminal Deprotonation.,Bacik JP, Mekasha S, Forsberg Z, Kovalevsky AY, Vaaje-Kolstad G, Eijsink VGH, Nix JC, Coates L, Cuneo MJ, Unkefer CJ, Chen JC Biochemistry. 2017 May 23;56(20):2529-2532. doi: 10.1021/acs.biochem.7b00019., Epub 2017 May 11. PMID:28481095<ref>PMID:28481095</ref>


Description: Room temperature X-ray crystallographic structure of a Jonesia denitrificans lytic polysaccharide monooxygenase at 1.1 angstrom resolution.
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Bacik, J.-P]]
<div class="pdbe-citations 5vg0" style="background-color:#fffaf0;"></div>
[[Category: Unkefer, C.J]]
== References ==
[[Category: Chen, J.C.H]]
<references/>
__TOC__
</StructureSection>
[[Category: Chitinase]]
[[Category: Bacik, J P]]
[[Category: Chen, J C.H]]
[[Category: Unkefer, C J]]
[[Category: Histidine brace]]
[[Category: Hydrolase]]
[[Category: Sugar binding protein]]
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