ConSurfDB vs. ConSurf: Difference between revisions
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Eric Martz (talk | contribs) |
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#Just below the large red line <font color="red">Please choose which sequences you want to use for ConSurf calculation</font> is a form. Put the number of the last sequence having the same function as the protein of interest in the box "Select the first [ .... ] sequences". Then click on the "Update selection" button. | #Just below the large red line <font color="red">Please choose which sequences you want to use for ConSurf calculation</font> is a form. Put the number of the last sequence having the same function as the protein of interest in the box "Select the first [ .... ] sequences". Then click on the "Update selection" button. | ||
## ConSurf will not accept >500 sequences. 200-250 sequences are plenty. More simply load the server unnecessarily. If the number of the last sequence having the same function as the query protein is higher than 250, use the radio buttons labeled "only every 2nd, 3rd, ..." to reduce the total number of sequences selected. | ## ConSurf will not accept >500 sequences. 200-250 sequences are plenty. More simply load the server unnecessarily. If the number of the last sequence having the same function as the query protein is higher than 250, use the radio buttons labeled "only every 2nd, 3rd, ..." to reduce the total number of sequences selected. | ||
The form is confusingly labeled. If you check an "only every" number, then you will need to divide the number in the slot labeled "Select the first" by the "only every" number you selected. For example, if the first 472 sequences have the same function as the query protein, check "only every 2nd" and enter 236 (namely, 472/2) in the "Select the first" slot. | ##The form is confusingly labeled. If you check an "only every" number, then you will need to divide the number in the slot labeled "Select the first" by the "only every" number you selected. For example, if the first 472 sequences have the same function as the query protein, check "only every 2nd" and enter 236 (namely, 472/2) in the "Select the first" slot. | ||
#Examine the list of sequences to make sure that only the desired sequences are checked. (Of course you may check or uncheck individual sequences if you wish.) | #Examine the list of sequences to make sure that only the desired sequences are checked. (Of course you may check or uncheck individual sequences if you wish.) | ||
#When you are satisfied, scroll to the very bottom of the page and click the ''Submit'' button. | #When you are satisfied, scroll to the very bottom of the page and click the ''Submit'' button. | ||
