1w0d: Difference between revisions
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK03437 PRK03437]</span> | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK03437 PRK03437]</span> | ||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w0d OCA], [http://www.ebi.ac.uk/pdbsum/1w0d PDBsum | |RELATEDENTRY= | ||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w0d OCA], [http://www.ebi.ac.uk/pdbsum/1w0d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1w0d RCSB]</span> | |||
}} | }} | ||
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[[Category: tbsgc]] | [[Category: tbsgc]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:29:24 2008'' | ||
Revision as of 21:29, 30 March 2008
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| 1w0d, resolution 1.65Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | SO4 | ||||||||||||
| Activity: | 3-isopropylmalate dehydrogenase, with EC number 1.1.1.85 | ||||||||||||
| Domains: | PRK03437 | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
THE HIGH RESOLUTION STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS LEUB (RV2995C)
Overview
The crystal structure of the enzyme 3-isopropylmalate dehydrogenase (IPMDH) from Mycobacterium tuberculosis (LeuB, Mtb-IPMDH, Rv2995c) without substrate or co-factor was determined at 1.65 A resolution, which is the highest resolution reported for an IPMDH to date. The crystals contain two functional dimers in the asymmetric unit in an arrangement close to a tetramer of D2 symmetry. Despite the absence of a substrate or inhibitor bound to the protein, the structure of the monomer resembles the previously observed closed form of the enzyme more closely than the open form. A comparison with the substrate complex of IPMDH from Thiobacillus ferrooxidans and the co-factor complex of the Thermus thermophilus enzyme revealed a close relationship of the active-site architecture between the various bacterial enzymes. The inhibitor O-isobutenyl oxalylhydroxamate was found to bind to the active site of IPMDH in a mode similar to the substrate isopropylmalate.
About this Structure
1W0D is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
Reference
The high-resolution Structure of LeuB (Rv2995c) from Mycobacterium tuberculosis., Singh RK, Kefala G, Janowski R, Mueller-Dieckmann C, von Kries JP, Weiss MS, J Mol Biol. 2005 Feb 11;346(1):1-11. Epub 2004 Dec 23. PMID:15663922
Page seeded by OCA on Mon Mar 31 00:29:24 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- 3-isopropylmalate dehydrogenase
- Mycobacterium tuberculosis
- Single protein
- Janowski, R.
- Kefala, G.
- Mueller-Dieckmann, C.
- Singh, R K.
- TBSGC, TB Structural Genomics Consortium.
- Weiss, M S.
- Dehydrogenase,oxidoreductase,leucine biosynthesis,nad
- Structural genomics,psi,protein structure initiative
- Tb structural genomics consortium,tb
- Tbsgc
