5w0t

Crystal structure of monomeric Msp1 from S. cerevisiae

spinqualitylabelsall models 5w0t, resolution 2.63Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5w0t is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO NonStd Res: MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [MSP1_YEAST] Involved in intramitochondrial sorting of proteins. Publication Abstract from PubMed Mislocalized tail-anchored (TA) proteins of the outer mitochondrial membrane are cleared by a newly identified quality control pathway involving the conserved eukaryotic protein Msp1 (ATAD1 in humans). Msp1 is a transmembrane AAA-ATPase, but its role in TA protein clearance is not known. Here, using purified components reconstituted into proteoliposomes, we show that Msp1 is both necessary and sufficient to drive the ATP-dependent extraction of TA proteins from the membrane. A crystal structure of the Msp1 cytosolic region modeled into a ring hexamer suggests that active Msp1 contains a conserved membrane-facing surface adjacent to a central pore. Structure-guided mutagenesis of the pore residues shows that they are critical for TA protein extraction in vitro and for functional complementation of an msp1 deletion in yeast. Together, these data provide a molecular framework for Msp1-dependent extraction of mislocalized TA proteins from the outer mitochondrial membrane. Msp1 Is a Membrane Protein Dislocase for Tail-Anchored Proteins.,Wohlever ML, Mateja A, McGilvray PT, Day KJ, Keenan RJ Mol Cell. 2017 Jul 20;67(2):194-202.e6. doi: 10.1016/j.molcel.2017.06.019. Epub, 2017 Jul 14. PMID:28712723 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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