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'''Unreleased structure'''


The entry 5vit is ON HOLD  until Paper Publication
==Crystal structure of a Pseudomonas malonate decarboxylase hetero-tetramer in complex with malonate==
<StructureSection load='5vit' size='340' side='right' caption='[[5vit]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5vit]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VIT FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5vip|5vip]], [[5vj1|5vj1]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malonyl-S-ACP:biotin-protein_carboxyltransferase Malonyl-S-ACP:biotin-protein carboxyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.10 2.1.3.10] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vit OCA], [http://pdbe.org/5vit PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vit RCSB], [http://www.ebi.ac.uk/pdbsum/5vit PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vit ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/MDCC_PSEF5 MDCC_PSEF5]] Subunit of malonate decarboxylase, it is an acyl carrier protein to which acetyl and malonyl thioester residues are bound via a 2'-(5''-phosphoribosyl)-3'-dephospho-CoA prosthetic group and turn over during the catalytic mechanism.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pseudomonas species and other aerobic bacteria have a biotin-independent malonate decarboxylase that is crucial for their utilization of malonate as the sole carbon and energy source. The malonate decarboxylase holoenzyme contains four subunits, having an acyl-carrier protein (MdcC subunit) with a distinct prosthetic group, as well as decarboxylase (MdcD-MdcE) and acyl-carrier protein transferase (MdcA) catalytic activities. Here we report the crystal structure of a Pseudomonas malonate decarboxylase hetero-tetramer, as well as biochemical and functional studies based on the structural information. We observe a malonate molecule in the active site of MdcA and we also determine the structure of malonate decarboxylase with CoA in the active site of MdcD-MdcE. Both structures provide molecular insights into malonate decarboxylase catalysis. Mutations in the hetero-tetramer interface can abolish holoenzyme formation. Mutations in the hetero-tetramer interface and the active sites can abolish Pseudomonas aeruginosa growth in a defined medium with malonate as the sole carbon source.Some aerobic bacteria contain a biotin-independent malonate decarboxylase (MDC), which allows them to use malonate as the sole carbon source. Here, the authors present the crystal structure of a Pseudomonas MDC and give insights into its catalytic mechanism and function.


Authors: Maderbocus, R., Tong, L.
Crystal structure of a Pseudomonas malonate decarboxylase holoenzyme hetero-tetramer.,Maderbocus R, Fields BL, Hamilton K, Luo S, Tran TH, Dietrich LEP, Tong L Nat Commun. 2017 Jul 31;8(1):160. doi: 10.1038/s41467-017-00233-z. PMID:28757619<ref>PMID:28757619</ref>


Description: Crystal structure of a Pseudomonas malonate decarboxylase hetero-tetramer in complex with malonate
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 5vit" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Malonyl-S-ACP:biotin-protein carboxyltransferase]]
[[Category: Maderbocus, R]]
[[Category: Tong, L]]
[[Category: Tong, L]]
[[Category: Maderbocus, R]]
[[Category: Acetyl-coa carboxylase]]
[[Category: Acyl carrier protein]]
[[Category: Transferase]]
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