1wac: Difference between revisions
No edit summary |
No edit summary |
||
| Line 7: | Line 7: | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wac OCA], [http://www.ebi.ac.uk/pdbsum/1wac PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wac RCSB]</span> | |||
}} | }} | ||
| Line 16: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
1WAC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | 1WAC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA]. | ||
==Reference== | ==Reference== | ||
Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15659773 15659773] | Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15659773 15659773] | ||
[[Category: | [[Category: Pseudomonas phage phi6]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bamford, D H.]] | [[Category: Bamford, D H.]] | ||
| Line 30: | Line 33: | ||
[[Category: transcription]] | [[Category: transcription]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:33:28 2008'' | ||
Revision as of 21:33, 30 March 2008
| |||||||||||||
| 1wac, resolution 3.0Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE
Overview
The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.
About this Structure
1WAC is a Single protein structure of sequence from Pseudomonas phage phi6. Full crystallographic information is available from OCA.
Reference
Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:15659773
Page seeded by OCA on Mon Mar 31 00:33:28 2008
