5osn

Crystal Structure of Bovine Enterovirus 2 determined with Serial Femtosecond X-ray Crystallography

spinqualitylabelsall models 5osn, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5osn is a 4 chain structure with sequence from Enterovirus e. This structure supersedes the now removed PDB entry 5mqu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GLU, K, OSF, SO4, SPH Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, we were able to determine the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin, with total data collection times of less than 14 and 10 min, respectively. Our method requires only micrograms of sample and should therefore broaden the applicability of serial femtosecond crystallography to challenging projects for which only limited sample amounts are available. By synchronizing the sample exchange to the XFEL repetition rate, our method allows for most efficient use of the limited beam time available at XFELs and should enable a substantial increase in sample throughput at these facilities. High-speed fixed-target serial virus crystallography.,Roedig P, Ginn HM, Pakendorf T, Sutton G, Harlos K, Walter TS, Meyer J, Fischer P, Duman R, Vartiainen I, Reime B, Warmer M, Brewster AS, Young ID, Michels-Clark T, Sauter NK, Kotecha A, Kelly J, Rowlands DJ, Sikorsky M, Nelson S, Damiani DS, Alonso-Mori R, Ren J, Fry EE, David C, Stuart DI, Wagner A, Meents A Nat Methods. 2017 Aug;14(8):805-810. doi: 10.1038/nmeth.4335. Epub 2017 Jun 19. PMID:28628129 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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